Abstract
The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays of intragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Davis, L., and Smith, G.R. (2003) Non-random homolog segregation at meiosis I in Schizosaccharomyces pombe mutants lacking recombination. Genetics 163, 857–874.
Cromie, G.A., and Smith, G.R. (2008) Meiotic recombination in Schizosaccharomyces pombe: A paradigm for genetic and molecular analysis, in “Genome Dynamics and Stability” (Egel, R. and Lankenau, D-H., eds.), Springer-Verlag, Berlin, pp. 195–230.
Wood, V., Gwilliam, R., Rajandream, M.-A., Lyne, M., Lyne, R., Stewart, A., Sgouros, J., Peat, N., Hayles, J., Baker, S., Basham, D., et al. (2002) The genome sequence of Schizosaccharomyces pombe. Nature 415, 871–880.
Moreno, S., Klar, A.J.S., and Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795–823.
Egel, R. (Ed.) (2004) The Molecular Biology of Schizosaccharomyces pombe. Springer-Verlag, Berlin.
Iino, Y., and Yamamoto, M. (1985) Mutants of Schizosaccharomyces pombe which sporulate in the haploid state. Mol. Gen. Genet. 198, 416–421.
Yamamoto, A., and Hiraoka, Y. (2003) Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast. EMBO J. 22, 2284–2296.
Tange, Y., Horio, T., Shimanuki, M., Ding, D.Q., Hiraoka, Y., and Niwa, O. (1998) A novel fission yeast gene, tht1 +, is required for the fusion of nuclear envelopes during karyogamy. J. Cell. Biol. 140, 247–258.
Martín-Castellanos, C., Blanco, M., Rozalén, A.E., Pérez-Hidalgo, L., García, A.I., Conde, F., Mata, J., Ellermeier, C., Davis, L., San-Segundo, P., Smith, G.R., and Moreno, S. (2005) A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr. Genet. 15, 2056–2062.
Li, Y.F., and Smith, G.R. (1997) The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events. Genetics 146, 57–67.
Niwa, O., and Yanagida, M. (1985) Triploid meiosis and aneuploidy in Schizosaccharomyces pombe: an unstable aneuploid disomic for chromosome III. Curr. Genet. 9, 463–470.
Young, J.A., Schreckhise, R.W., Steiner, W.W., and Smith, G.R. (2002) Meiotic recombination remote from prominent DNA break sites in S. pombe. Mol. Cell 9, 253–263.
Haldane, J.B.S. (1919) The combination of linkage values, and the calculation of distances between the loci of linked factors. J. Genet. 8, 299–309.
Perkins, D.D. (1949) Biochemical mutants in the smut fungus Ustilago maydis. Genetics 34, 607–626.
Munz, P. (1994) An analysis of interference in the fission yeast Schizosaccharomyces pombe. Genetics 137, 701–707.
Acknowledgments
I am grateful to Sue Amundsen, Gareth Cromie, and Luther Davis for helpful comments on the manuscript. My laboratory is supported by research grants GM031693 and GM032194 from the National Institutes of Health.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Smith, G.R. (2009). Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe . In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 557. Humana Press. https://doi.org/10.1007/978-1-59745-527-5_6
Download citation
DOI: https://doi.org/10.1007/978-1-59745-527-5_6
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-934115-66-4
Online ISBN: 978-1-59745-527-5
eBook Packages: Springer Protocols