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GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling

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Hedgehog Signaling Protocols

Part of the book series: Methods Inmolecular Biology™ ((MIMB,volume 397))

Abstract

The GAL4/upstream activating sequence (UAS) system is one of the most powerful tools for targeted gene expression. It is based on the properties of the yeast GAL4 transcription factor which activates transcription of its target genes by binding to UAS cis-regulatory sites. In Drosophila, the two components are carried in separate lines allowing for numerous combinatorial possibilities. The driver lines provide tissue-specific GAL4 expression and the responder lines carry the coding sequence for the gene of interest under the control of UAS sites. In this chapter, the basic GAL4/UAS system and its extensions, namely those allowing precise temporal control and reversible expression, are described. In addition, a list of GAL4 and UAS lines and schematic maps of GAL4 and UAS vectors useful in the study of Hedgehog (Hh) signaling is given. Finally, uses of the GAL4/UAS system to resolve some of the questions addressed in the study of the Hh pathway are presented.

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Authors and Affiliations

  1. Institut Jacques Monod (UMR7592-CNRS/Universités Paris VI et VII), Tour 42 5éme étage 2-4, Place Jussieu, Paris Cedex, France

    Denise Busson

  2. Centre de Génétique Moléculaire (UPR2167-CNRS associé à l’Université Paris VI), de la Terrasse Gif sur Yvette, France

    Anne-Marie Pret

Authors
  1. Denise Busson
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  2. Anne-Marie Pret
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Editors and Affiliations

  1. Deptment of Biomedical Sciences College of Medicine, Florida State University, Tallahassee, Florida, USA

    Jamila I. Horabin (Associate Professor) (Associate Professor)

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© 2007 Humana Press Inc., Totowa, NJ

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Busson, D., Pret, AM. (2007). GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling. In: Horabin, J.I. (eds) Hedgehog Signaling Protocols. Methods Inmolecular Biology™, vol 397. Humana Press. https://doi.org/10.1007/978-1-59745-516-9_13

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  • DOI: https://doi.org/10.1007/978-1-59745-516-9_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-692-4

  • Online ISBN: 978-1-59745-516-9

  • eBook Packages: Springer Protocols

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