Abstract
Improved methods of baculovirus cloning and insect cell culture and their commercialization have made the use of the baculovirus expression vector system (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and expression optimization.
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© 2007 Humana Press Inc., Totowa, NJ
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Harwood, S. (2007). Small-Scale Protein Production With the Baculovirus Expression Vector System. In: Murhammer, D.W. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology™, vol 388. Humana Press. https://doi.org/10.1007/978-1-59745-457-5_10
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DOI: https://doi.org/10.1007/978-1-59745-457-5_10
Publisher Name: Humana Press
Print ISBN: 978-1-58829-537-8
Online ISBN: 978-1-59745-457-5
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