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Baculovirus and Insect Cell Expression Protocols

  • David W. Murhammer

Part of the Methods in Molecular Biology™ book series (MIMB, volume 388)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

    1. Front Matter
      Pages 23-23
    2. Barbara J. Kelly, Linda A. King, Robert D. Possee
      Pages 25-53
    3. Robert D. Possee, Linda A. King
      Pages 55-75
    4. Linda A. King, Richard Hitchman, Robert D. Possee
      Pages 77-93
    5. James A. Fornwald, Quinn Lu, Dayuan Wang, Robert S. Ames
      Pages 95-114
  4. Insect Cell Culture

  5. Protein Production with Recombinant Baculoviruses

    1. Front Matter
      Pages 209-209
    2. Sue H. Kadwell, Philip I. Hardwicke
      Pages 247-266
    3. Yi Liu, Nathan De Carolis, Nikolai van Beek
      Pages 267-279
    4. Marc G. Aucoin, Danielle Jacob, Parminder Singh Chahal, Jamal Meghrous, Alice Bernier, Amine A. Kamen
      Pages 281-296
  6. Recombinant Protein Production with Transformed Insect Cells

    1. Front Matter
      Pages 297-297
    2. Lucy Cherbas, Peter Cherbas
      Pages 317-340
    3. Robert L. Harrison, Donald L. Jarvis
      Pages 341-356
  7. Baculovirus Development and Production for Use as Insecticides

    1. Front Matter
      Pages 357-357
    2. Bryony C. Bonning, Tyasning Nusawardani
      Pages 359-366
    3. Nikolai van Beek, David C. Davis
      Pages 367-378
  8. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

    1. Front Matter
      Pages 405-405
    2. Yu-Chen Hu, William E. Bentley
      Pages 419-425
    3. John C. March, William E. Bentley
      Pages 427-433
  9. Back Matter
    Pages 461-470

About this book

Introduction

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

Keywords

Expression Protein cell lines gene expression genes molecular biology proteins

Editors and affiliations

  • David W. Murhammer
    • 1
  1. 1.Department of Chemical and Biochemical EngineeringThe University of IowaIowa City

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-457-5
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-537-8
  • Online ISBN 978-1-59745-457-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site