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Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration

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Extracellular Matrix Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 522))

Summary

Cell adhesion and cell migration are two primary cellular phenomena for which in vitro approaches may be exploited to effectively dissect the individual events and underlying molecular mechanisms. The use of assays dedicated to the analysis of cell adhesion and migration in vitro also afford an efficient way of conducting larger basic and applied research screenings on the factors affecting these processes and are potentially exploitable in the context of routine diagnostic, prognostic, and predictive tests in the biological and medical fields. Therefore, there is a longstanding continuum in the interest in devising more rationale such assays and major contributions in this direction have been provided by the advent of procedures based on fluorescence cell tagging, the design of instruments capable of detecting fluorescent signals with high sensitivity, and informatic tools allowing sophisticated elaboration of data generated through these instruments. In this report, we describe three representative fluorescence-based model assays for the qualitative and quantitative assessment of cell adhesion and cell locomotion in static and dynamic conditions. The assays are easily performed, accurate and reproducible, and can be automated for high-to-medium throughput screenings of cell behavior in vitro. Performance of the assays involves the use of certain dedicated disposable accessories, which are commercially available, and a few instruments that, due to their versatility, can be regarded as constituents of a more generic laboratory setup.

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Acknowledgments

We are grateful for the financial support from the Italian Ministry of Health and the Italian Ministry of University, Associazione Italiana per la Ricerca sul Cancro (AIRC), ABO Project 2010, Mizutani Glycoscience Foundation (Japan) and research funds from the University of Parma. We are also grateful to a number of collaborators that during the years have assisted us in the development and refinement of the cell adhesion and migration assays described in this article.

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Authors and Affiliations

  1. Department of Geneticsbiology and Anthropology, University of Parma, Via G.P. Usberti 11/A, IT-43100, Parma, Italy

    Roberto Perris

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  1. Paola Spessotto
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  2. Katia Lacrima
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  3. Pier Andrea Nicolosi
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  4. Eliana Pivetta
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  5. Martina Scapolan
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  6. Roberto Perris
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Correspondence to Roberto Perris .

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Editors and Affiliations

  1. Gene Therapy Inst., Hadassah Univ. Hospital, Jerusalem, 91 120, Israel

    Sharona Even-Ram

  2. Lombardi Comprehensive Cancer Ctr., Georgetown University, Reservoir Rd. NW., 3970, Washington, 20057-1468, U.S.A.

    Vira Artym

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Spessotto, P., Lacrima, K., Nicolosi, P., Pivetta, E., Scapolan, M., Perris, R. (2009). Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration. In: Even-Ram, S., Artym, V. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology, vol 522. Humana Press. https://doi.org/10.1007/978-1-59745-413-1_16

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  • DOI: https://doi.org/10.1007/978-1-59745-413-1_16

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