Abstract
Rodent arthritis models are tools to study joint destruction. This chapter describes methods to prepare and analyze histological sections from inflamed paws. Protocols to create decalcified paraffin-embedded as well as undecalcified plastic-embedded sections are provided. Strengths and limitations of these two histological techniques are described. Major staining techniques on hard tissue sections focusing on the evaluation of joint destruction are indicated. Finally, evaluation of arthritic paw sections by histomorphometry, a semiautomated computerized method to measure areas of interest, is described.
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© 2007 Humana Press Inc., Totowa, NJ
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Schett, G., Tuerk, B. (2007). Bone Histomorphometry in Arthritis Models. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 135. Humana Press. https://doi.org/10.1007/978-1-59745-401-8_17
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DOI: https://doi.org/10.1007/978-1-59745-401-8_17
Publisher Name: Humana Press
Print ISBN: 978-1-58829-344-2
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