Abstract
This chapter describes a luciferase-based protocol to measure adenosine triphosphate (ATP) production in isolated mitochondria of Trypanosoma brucei. The assay represents an excellent method to characterize the functionality of isolated mitochondria. Comparing the ATP production induced by substrates for oxidative phosphorylation to the one induced by substrates for substrate-level phosphorylation allows conclusions regarding the integrity of the outer and inner mitochondrial membranes. Furthermore, the assay is a valuable tool for characterization of RNA interference cell lines suspected to affect mitochondrial functions.
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© 2007 Humana Press Inc., Totowa, NJ
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Schneider, A., Bouzaidi-Tiali, N., Chanez, AL., Bulliard, L. (2007). ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei . In: Leister, D., Herrmann, J.M. (eds) Mitochondria. Methods in Molecular Biology™, vol 372. Humana Press. https://doi.org/10.1007/978-1-59745-365-3_27
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DOI: https://doi.org/10.1007/978-1-59745-365-3_27
Publisher Name: Humana Press
Print ISBN: 978-1-58829-667-2
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