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Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections

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Electron Microscopy

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 369))

Abstract

In correlative microscopy, light microscopy provides the overview and orientation in the complex cells and tissue, whereas electron microscopy offers the detailed localization and correlation to subcellular structures. In this chapter, we offer the detailed high-quality electron microscopical preparation methods for the optimum preservation of the cellular ultrastructure. From such preparations, serial thin sections are collected and used for comparative histochemical, immunofluorescence, and immunogold staining. In light microscopy, histological stains are used to identify the orientation of the sample, and immunofluorescence labeling is used to identify the region of interest, namely, the labeled cells expressing the macromolecule under investigation. Subsequent sections, labeled with immunogold, are analyzed by electron microscopy to identify the label within the cellular architecture at high resolution.

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Authors and Affiliations

  1. Max-Planck-Institut für Entwicklungsbiologie, Tuebingen, Germany

    Heinz Schwarz

  2. Electron Microscopy and Structural Analysis, Cellular Architecture and Dynamics, Department of Biology, Faculty of Sciences, Utrecht University, Utrecht, The Netherlands

    Bruno M. Humbel

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  1. Heinz Schwarz
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  1. Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Western Australia, Australia

    John Kuo

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Schwarz, H., Humbel, B.M. (2007). Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections. In: Kuo, J. (eds) Electron Microscopy. Methods in Molecular Biology™, vol 369. Humana Press. https://doi.org/10.1007/978-1-59745-294-6_12

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  • DOI: https://doi.org/10.1007/978-1-59745-294-6_12

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