Electron Microscopy

Methods and Protocols

  • John Kuo

Part of the Methods in Molecular Biology™ book series (MIMB, volume 369)

Table of contents

  1. Front Matter
    Pages i-xv
  2. José A. Mascorro, John J. Bozzola
    Pages 19-34
  3. Kent L. McDonald, Mary Morphew, Paul Verkade, Thomas Müller-Reichert
    Pages 143-173
  4. Dimitri Vanhecke, Luca Studer, Daniel Studer
    Pages 175-197
  5. Margaret Coughlin, William M. Brieher, Ryoma Ohi
    Pages 199-212
  6. Dušan Cmarko, Karel Koberna
    Pages 213-228
  7. Paul A. Sims, Jeff D. Hardin
    Pages 291-308
  8. Terry M. Mayhew
    Pages 309-329
  9. Hui-Ting Chou, James E. Evans, Henning Stahlberg
    Pages 331-343
  10. Wen Jiang, Wah Chiu
    Pages 345-363
  11. Peter Engelhardt, Jari Meriläinen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui, Veli-Pekka Lehto
    Pages 387-405

About this book

Introduction

In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

Keywords

DNA biological materials electron microscopy hybridization membrane proteins molecular biology scanning electron microscope transmission electron microscopy

Editors and affiliations

  • John Kuo
    • 1
  1. 1.Centre for Microscopy and MicroanalysisThe University of Western AustraliaCrawleyAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-294-6
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-573-6
  • Online ISBN 978-1-59745-294-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book