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Electron Microscopy In Situ Hybridization

Tracking of DNA and RNA Sequences at High Resolution

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Electron Microscopy

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 369))

Abstract

Electron microscopy in situ hybridization (EM-ISH) represents a powerful method that enables the localization of specific sequences of nucleic acids at high resolution. We provide here an overview of three different nonisotopic EM-ISH approaches that allow the visualization of nucleic acid sequences in cells. A comparison of various methods with respect to their sensitivity and the structural preservation of the sample is presented, with the aim of helping the reader to choose a convenient hybridization procedure. The postembedding EM-ISH protocol that currently represents the most widely used technique is described in detail, with a special emphasis on the organization of the cell nucleus.

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Author information

Authors and Affiliations

  1. Institute of Cellular Biology and Pathology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

    Dušan Cmarko

  2. Department of Cell Biology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Karel Koberna

  3. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Karel Koberna

  4. Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Karel Koberna

Authors
  1. Dušan Cmarko
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  2. Karel Koberna
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Editor information

Editors and Affiliations

  1. Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Western Australia, Australia

    John Kuo

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© 2007 Humana Press Inc.

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Cmarko, D., Koberna, K. (2007). Electron Microscopy In Situ Hybridization. In: Kuo, J. (eds) Electron Microscopy. Methods in Molecular Biology™, vol 369. Humana Press. https://doi.org/10.1007/978-1-59745-294-6_11

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  • DOI: https://doi.org/10.1007/978-1-59745-294-6_11

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-573-6

  • Online ISBN: 978-1-59745-294-6

  • eBook Packages: Springer Protocols

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