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Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells

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Electron Microscopy

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 369))

Abstract

This chapter covers the conventional methods and considerations for preparing cultured cells for examination in the transmission electron microscope. Techniques for handling cells grown in liquid culture, as well as on substrates such as culture dishes or agar, are discussed. Directions are given on how to prepare the most commonly used buffers, fixatives, enrobement media, and embedding resins. These methods may be applied to most cultured organisms, from bacteria to mammalian cells.

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References

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Author information

Authors and Affiliations

  1. IMAGE Center, Southern Illinois University, Carbondale, IL

    John J. Bozzola

Authors
  1. John J. Bozzola
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Editor information

Editors and Affiliations

  1. Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Western Australia, Australia

    John Kuo

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© 2007 Humana Press Inc.

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Bozzola, J.J. (2007). Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells. In: Kuo, J. (eds) Electron Microscopy. Methods in Molecular Biology™, vol 369. Humana Press. https://doi.org/10.1007/978-1-59745-294-6_1

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  • DOI: https://doi.org/10.1007/978-1-59745-294-6_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-573-6

  • Online ISBN: 978-1-59745-294-6

  • eBook Packages: Springer Protocols

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