Abstract
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jin Y. (1999). Transformation. In: C. elegans: A Practical Approach. Hope I.A, ed. New York: Oxford University Press, 69–96.
Mello C., Fire A. (1995) DNA transformation. Meth. Cell Biol. 48, 451–82.
Kimble J., Hodgkin J., Smith T., Smith J. (1982) Suppression of an amber mutation by microinjection of suppressor tRNA in C. elegans. Nature 299, 456–8.
Stinchcomb D.T., Shaw J.E., Carr S.H., Hirsh D. (1985) Extrachromosomal DNA transformation of Caenorhabditis elegans. Mol. Cell Biol. 5, 3484–96.
Mello C.C., Kramer J.M., Stinchcomb D., Ambros V. (1991) Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 10, 3959–70.
Fire A. (1986). Integrative transformation of Caenorhabditis elegans. EMBO J. 5(10), 2673–80.
Kelly W.G., Xu S., Montgomery M.K., Fire A. (1997) Distinct requirements for somatic and germline expression of a generally expressed Caenorhabditis elegans gene. Genetics. 146, 227–38.
Praitis V., Casey E., Collar D., Austin J. (2001) Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics. 157(3), 1217–26.
Yochem J., Herman R.K. (2003) Investigating C. elegans development through mosaic analysis. Development. 130(20), 4761–8.
Brenner S. (1974) The genetics of Caenorhabditis elegans. Genetics. 77, 71–94.
Acknowledgments
We would like to thank members of the Singson lab for helpful comments and discussion. Research in the Singson lab has been supported by grants from the National Institutes of Health, the National Science Foundation, the Busch Biomedical Fund, and Johnson and Johnson Discovery Awards.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Kadandale, P., Chatterjee, I., Singson, A. (2009). Germline Transformation of Caenorhabditis elegans by Injection. In: Carroll, D. (eds) Microinjection. Methods in Molecular Biology, vol 518. Humana Press. https://doi.org/10.1007/978-1-59745-202-1_10
Download citation
DOI: https://doi.org/10.1007/978-1-59745-202-1_10
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-58829-884-3
Online ISBN: 978-1-59745-202-1
eBook Packages: Springer Protocols