Microinjection

Methods and Applications

  • David J. Carroll

Part of the Methods in Molecular Biology book series (MIMB, volume 518)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Karl Swann, Karen Campbell, Yuansong Yu, Christopher Saunders, F. Anthony Lai
    Pages 17-29
  3. Sebastien Chenuet, Madiha Derouazi, David Hacker, Florian Wurm
    Pages 99-112
  4. Pavan Kadandale, Indrani Chatterjee, Andrew Singson
    Pages 123-133
  5. Laurinda A. Jaffe, Rachael P. Norris, Marina Freudzon, William J. Ratzan, Lisa M. Mehlmann
    Pages 157-173
  6. Vincenzo Cavalieri, Maria Di Bernardo, Giovanni Spinelli
    Pages 175-188
  7. Satoshi Kishigami, Teruhiko Wakayama
    Pages 207-218
  8. Back Matter
    Pages 219-221

About this book

Introduction

As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.

Keywords

Antibodies Expression Gene function Nucleotide Protein expression RNA expression Regulation gene expression transgen

Editors and affiliations

  • David J. Carroll
    • 1
  1. 1.Dept. Biological SciencesFlorida Institute of TechnologyMelbourneU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-202-1
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-884-3
  • Online ISBN 978-1-59745-202-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book