Abstract
Human embryonic stem cells (HESCs) are widely used as a model system for human cell type specification. Genetic modification forms a valuable tool for HESC technology, as it provides the basis for lineage selection, i.e., the purification of a specific cell type after differentiation. Electroporation is an efficient way to transfect HESCs. Nucleofection is an electroporation-based transfection technique which utilizes cell-type-specific buffer solutions and specific electric settings. Customization of these two parameters has been proven to result in highly efficient gene transfer even in hard-to-transfect cells. We can show that nucleofection surpasses conventional electroporation in efficiency and decreases the experimental effort for transfection of HESCs.
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© 2008 Humana Press
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Siemen, H., Nolden, L., Terstegge, S., Koch, P., Brüstle, O. (2008). Nucleofection of Human Embryonic Stem Cells. In: Li, S. (eds) Electroporation Protocols. Methods in Molecular Biology™, vol 423. Humana Press. https://doi.org/10.1007/978-1-59745-194-9_8
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DOI: https://doi.org/10.1007/978-1-59745-194-9_8
Publisher Name: Humana Press
Print ISBN: 978-1-58829-877-5
Online ISBN: 978-1-59745-194-9
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