Electroporation Protocols

Preclinical and Clinical Gene Medicine

  • Shulin Li

Part of the Methods in Molecular Biology™ book series (MIMB, volume 423)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Basic Concepts of DNA Transfer via Electroporation

  3. In Vitro Targeted Gene Delivery via Electroporation

    1. Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, Oliver Brüstle
      Pages 131-138
    2. Linda N. Liu, Rama Shivakumar, Cornell Allen, Joseph C. Fratantoni
      Pages 139-153
    3. Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, Kris Thielemans
      Pages 155-163
    4. Kathrin Schoenberg, Hans-Ingo Trompeter, Markus Uhrberg
      Pages 165-172
    5. Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, Rozanne Arulanandam et al.
      Pages 173-189
  4. In Vivo Targeted Gene Delivery via Electroporation

    1. Capucine Trollet, Daniel Scherman, Pascal Bigey
      Pages 199-214
    2. Patricia A. Brown, Amir S. Khan, Ruxandra Draghia-Akli
      Pages 215-224
    3. Babu M. Medi, Jagdish Singh
      Pages 225-232
    4. Rui Zhou, James E. Norton, David A. Dean
      Pages 233-247
    5. Masaki Yoshida, Hitoshi Iwashita, Masayuki Otani, Koichi Masunaga, Akito Inadome
      Pages 249-257
    6. Takahiko Matsuda, Constance L. Cepko
      Pages 259-278

About this book


At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields.  In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease.  Following the successful Methods in Molecular Biologyseries format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.

Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.


DNA Herman RNA gene therapy genes molecular biology signal transduction

Editors and affiliations

  • Shulin Li
    • 1
  1. 1.Department of Comparative Biomedical SciencesSchool of Veterinary Medicine, Louisiana State UniversityBaton RougeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-194-9
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-877-5
  • Online ISBN 978-1-59745-194-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book