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Electroporation Protocols

Preclinical and Clinical Gene Medicine

  • Shulin Li

Part of the Methods in Molecular Biology™ book series (MIMB, volume 423)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Basic Concepts of DNA Transfer via Electroporation

  3. In Vitro Targeted Gene Delivery via Electroporation

    1. Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, Oliver Brüstle
      Pages 131-138
    2. Linda N. Liu, Rama Shivakumar, Cornell Allen, Joseph C. Fratantoni
      Pages 139-153
    3. Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, Kris Thielemans
      Pages 155-163
    4. Kathrin Schoenberg, Hans-Ingo Trompeter, Markus Uhrberg
      Pages 165-172
    5. Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, Rozanne Arulanandam et al.
      Pages 173-189
  4. In Vivo Targeted Gene Delivery via Electroporation

    1. Capucine Trollet, Daniel Scherman, Pascal Bigey
      Pages 199-214
    2. Patricia A. Brown, Amir S. Khan, Ruxandra Draghia-Akli
      Pages 215-224
    3. Babu M. Medi, Jagdish Singh
      Pages 225-232
    4. Rui Zhou, James E. Norton, David A. Dean
      Pages 233-247
    5. Masaki Yoshida, Hitoshi Iwashita, Masayuki Otani, Koichi Masunaga, Akito Inadome
      Pages 249-257
    6. Takahiko Matsuda, Constance L. Cepko
      Pages 259-278
    7. N. Madhusudhana Rao, K. Murali Rambabu, S. Harinarayana Rao
      Pages 289-298
  5. Treatment of Cancer via Electroporation Gene Therapy

  6. Treatment of Other Diseases via Electroporation Gene Therapy

    1. Jason G. Fewell
      Pages 375-382
    2. Guy P. Marti, Parsa Mohebi, Lixin Liu, Jiaai Wang, Tomoharu Miyashita, John W. Harmon
      Pages 383-391
    3. Kazuo Komamura, Jun-ichi Miyazaki, Enyu Imai, Kunio Matsumoto, Toshikazu Nakamura, Masatsugu Hori
      Pages 393-404
    4. Robert M.I. Kapsa, Sharon H.A. Wong, Anita F. Quigley
      Pages 405-419
    5. Kim E. Wells, Jill McMahon, Helen Foster, Aurora Ferrer, Dominic J. Wells
      Pages 421-431
    6. Vadim V. Fedorov, Vladimir P. Nikolski, Igor R. Efimov
      Pages 433-448
    7. Ruxandra Draghia-Akli, Amir S. Khan
      Pages 449-460
  7. Applications of Electroporation for DNA Vaccination

    1. Anna-Karin Roos, Alan King, Pavel Pisa
      Pages 463-472
    2. Arianna Smorlesi, Francesca Papalini, Sara Pierpaoli, Mauro Provinciali
      Pages 473-485
    3. Yong-Gang Zhao, Yuhong Xu
      Pages 487-495
    4. Torunn Elisabeth Tjelle, Dietmar Rabussay, Christian Ottensmeier, Iacob Mathiesen, Rune Kjeken
      Pages 497-507
    5. Kaw Yan Chua, John D.A. Ramos, Nge Cheong
      Pages 509-520
  8. Back Matter
    Pages 521-524

About this book

Introduction

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields.  In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease.  Following the successful Methods in Molecular Biologyseries format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.

Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.

Keywords

DNA Herman RNA gene therapy genes molecular biology signal transduction

Editors and affiliations

  • Shulin Li
    • 1
  1. 1.Department of Comparative Biomedical SciencesSchool of Veterinary Medicine, Louisiana State UniversityBaton RougeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-194-9
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-877-5
  • Online ISBN 978-1-59745-194-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site