Abstract
Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -lglutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.
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References
D’Mello, S. R., Galli, C., Ciotti, T., and Calissano, P. (1993) Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP. Proc. Natl. Acad. Sci. USA 90(23), 10989–10993.
Gallo, V., Kingsbury, A., Balazs, R., and Jorgensen, O. S. (1987) The role of depolarization in the survival and differentiation of cerebellar granule cells in culture. J. Neurosci. 7(7), 2203–2213.
Franklin, J. L., and Johnson, E. M. (1992) Suppression of programmed neuronal death by sustained elevation of cytoplasmic calcium. Trends Neurosci. 15(12), 501–508.
See, V., Boutillier, A. L., Bito, H., and Loeffler, J. P. (2001) Calcium/calmodulin-dependent protein kinase type IV (CaMKIV) inhibits apoptosis induced by potassium deprivation in cerebellar granule neurons. FASEB J. 15(1), 134–144.
Acknowledgements
The authors would like to thank Dr. Armando A. Genazzani for passing on his knowledge and experiences with this method.
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Krämer, D., Minichiello, L. (2010). Cell Culture of Primary Cerebellar Granule Cells. In: Ward, A., Tosh, D. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 633. Humana Press. https://doi.org/10.1007/978-1-59745-019-5_17
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DOI: https://doi.org/10.1007/978-1-59745-019-5_17
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