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Mouse Cell Culture

Methods and Protocols

  • Andrew Ward
  • David Tosh

Part of the Methods in Molecular Biology book series (MIMB, volume 633)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, Lesley M. Forrester
    Pages 1-18
  3. Jonathan M. Quinlan, Wei-Yuan Yu, David Tosh
    Pages 81-90
  4. Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, David Tosh
    Pages 91-99
  5. Antonio Musarò, Laura Barberi
    Pages 101-111
  6. Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, Paul J.R. Barton
    Pages 113-124
  7. Wan-Chun Li, Kate L. Ralphs, David Tosh
    Pages 185-196
  8. Charles Frederick Campbell
    Pages 197-206
  9. Trevelyan R. Menheniott, Marika Charalambous, Andrew Ward
    Pages 207-220
  10. Dana Krämer, Liliana Minichiello
    Pages 233-239
  11. Back Matter
    Pages 253-257

About this book

Introduction

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Keywords

Cultured cells Developmental processes Fetal tissues Genetic animal model Homogeneous cell populations Isolated cell types Stem cells Vivo molecular biology

Editors and affiliations

  • Andrew Ward
    • 1
  • David Tosh
    • 2
  1. 1.Dept. Biology & BiochemistryUniversity of BathBathUnited Kingdom
  2. 2.Dept. Biology & BiochemistryUniversity of BathBathUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-019-5
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-772-3
  • Online ISBN 978-1-59745-019-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site