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Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation

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X-Chromosome Inactivation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1861))

Abstract

A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.

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Acknowledgments

We thank past and present members of the Namekawa laboratory for optimizing the experimental conditions. We thank Anna Sosso for reviewing the chapter. This work was supported by the Albert J. Ryan Fellowship to K.G.A. and the NIH Grant GM098605 to S.H.N.

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Authors and Affiliations

  1. Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA

    Kris G. Alavattam, Hironori Abe, Akihiko Sakashita & Satoshi H. Namekawa

  2. Division of Developmental Biology, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA

    Kris G. Alavattam, Hironori Abe, Akihiko Sakashita & Satoshi H. Namekawa

  3. Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA

    Kris G. Alavattam, Hironori Abe, Akihiko Sakashita & Satoshi H. Namekawa

Authors
  1. Kris G. Alavattam
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  2. Hironori Abe
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  3. Akihiko Sakashita
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  4. Satoshi H. Namekawa
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Corresponding authors

Correspondence to Kris G. Alavattam or Satoshi H. Namekawa .

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Editors and Affiliations

  1. Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, Japan

    Takashi Sado

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Alavattam, K.G., Abe, H., Sakashita, A., Namekawa, S.H. (2018). Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation. In: Sado, T. (eds) X-Chromosome Inactivation. Methods in Molecular Biology, vol 1861. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8766-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8766-5_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8765-8

  • Online ISBN: 978-1-4939-8766-5

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