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Genetic Transformation of Pentalinon andrieuxii Tissue Cultures

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Pentalinon andrieuxii is a species used in Mayan traditional medicine due to its biological properties. Recent studies indicate that it produces a pentacyclic triterpene-denominated betulinic acid, which presents various biological activities: antibacterial, antifungal, antiplasmodial, anti-inflammatory, antimalarial, anticancer, leishmanicidal, and antiviral, as well as steroids and sterols with leishmanicidal properties. A recent study also reported the presence of urechitol A and B in the roots; these are secondary metabolites whose biochemical function is as yet unknown. This plant therefore represents a natural source of metabolites with potential application in the pharmaceutical industry. In this chapter, a protocol is described for obtaining transgenic plants, at the reporter gene of the β-glucuronidase (GUS) via Agrobacterium tumefaciens from hypocotyl and root explants. The protocol established herein could be employed for the manipulation of the genes involved in the biosynthesis of isoprenoids or secondary metabolites of interest. To our knowledge, this is the first report of stable transformation of Pentalinon andrieuxii via Agrobacterium tumefaciens.

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Acknowledgment

The authors would like to express their gratitude to the Consejo Nacional de Ciencia y Tecnología (CONACYT) of Mexico for the financial support received for the projects 223404 and 257915. Our thanks also for the Master’s scholarship (280663) awarded to Yeseña Beatriz Burgos May.

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Authors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Yeseña Burgos-May & Elidé Avilés-Berzunza

  2. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Luis Manuel Peña-Rodríguez

  3. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México

    Gregorio Godoy-Hernández

Authors
  1. Yeseña Burgos-May
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  2. Elidé Avilés-Berzunza
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  3. Luis Manuel Peña-Rodríguez
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  4. Gregorio Godoy-Hernández
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Corresponding author

Correspondence to Gregorio Godoy-Hernández .

Editor information

Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Burgos-May, Y., Avilés-Berzunza, E., Peña-Rodríguez, L.M., Godoy-Hernández, G. (2018). Genetic Transformation of Pentalinon andrieuxii Tissue Cultures. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_33

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_33

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8593-7

  • Online ISBN: 978-1-4939-8594-4

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