Abstract
To get a complete understanding of cell migration, it is critical to study its orchestration at the molecular level. Since the recent developments in single-molecule imaging, it is now possible to study molecular phenomena at the single-molecule level inside living cells. In this chapter, we describe how such approaches have been and can be used to decipher molecular mechanisms involved in cell migration.
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Abbreviations
- AOTF:
-
Acousto-optic tunable filter
- Cas9:
-
CRISPR associated protein 9
- CRISPR:
-
Clustered regularly interspaced short palindromic repeats
- D :
-
Diffusion coefficient
- DMEM:
-
Dulbecco’s modified Eagle medium, high glucose
- EDTA:
-
Ethylenediaminetetraacetic acid
- EMCCD:
-
Electron-multiplying charge-coupled device
- FBS:
-
Fetal bovine serum
- FWHM :
-
Full width at half maximum
- GFP:
-
Green fluorescent protein
- MEF:
-
Mouse embryonic fibroblast
- mEos2:
-
Monomeric Eos2
- MSD:
-
Mean square displacement
- NA:
-
Numerical aperture
- PALM:
-
Photoactivation localization microscopy
- PBS:
-
Phosphate buffered saline
- sCMOS:
-
Scientific complementary metal-oxide semiconductor
- SRR:
-
Superresolved reconstruction image
- STL:
-
Superresolution time-lapse movie
- TIRF:
-
Total internal reflection fluorescence
- uPAINT:
-
Universal point accumulation imaging in nanoscale topography
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Orré, T., Mehidi, A., Massou, S., Rossier, O., Giannone, G. (2018). Using Single-Protein Tracking to Study Cell Migration. In: Gautreau, A. (eds) Cell Migration. Methods in Molecular Biology, vol 1749. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7701-7_21
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DOI: https://doi.org/10.1007/978-1-4939-7701-7_21
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