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Quantifying Alterations in Cell Migration: Tracking Fluorescently-Tagged Migrating Cells by FACs and Live-Imaging

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Wound Regeneration and Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1037))

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Abstract

Cell migration is fundamental to many different physiological processes including embryonic development, inflammation and wound healing. Given the range and importance cell migration plays a number of assays have been developed to measure different aspects of cell migration. Here we describe two different methods to analyze cell migration. The first method analyzes the migration of fluorescently tagged cells using Boyden chambers and FACs and the second looks at migration properties using time-lapse microscopy.

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Author information

Authors and Affiliations

  1. Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia

    Rachael Z. Murray

Authors
  1. Rachael Z. Murray
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Editor information

Editors and Affiliations

  1. Virginia Tech Carilion Research Institut, Roanoke, Virginia, USA

    Robert G. Gourdie

  2. School of Pharmacy, MCPHS University, Worcester, Massachusetts, USA

    Tereance A. Myers

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Murray, R.Z. (2013). Quantifying Alterations in Cell Migration: Tracking Fluorescently-Tagged Migrating Cells by FACs and Live-Imaging. In: Gourdie, R., Myers, T. (eds) Wound Regeneration and Repair. Methods in Molecular Biology, vol 1037. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-505-7_28

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  • DOI: https://doi.org/10.1007/978-1-62703-505-7_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-504-0

  • Online ISBN: 978-1-62703-505-7

  • eBook Packages: Springer Protocols

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