Abstract
In this chapter, we describe a stepwise protocol of microinjection. Using this method, antisense morpholinos, CRISPR-Cas9 ribonucleoprotein complexes, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.
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Acknowledgments
We thank Ms. Allison Malick, University of Michigan, for proofreading this manuscript. This work was supported by National Science Foundation grant IOS-1557850 and University of Michigan MCube2.0 Project U0496246 to CD.
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Xin, Y., Duan, C. (2018). Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_18
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DOI: https://doi.org/10.1007/978-1-4939-7665-2_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7664-5
Online ISBN: 978-1-4939-7665-2
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