Abstract
In pancreatic cancer, neural invasion is one of the most common paths of cancer dissemination. Classically, cancer cells actively invade nerves and cause local recurrence and pain. Three-dimensional (3D) neural migration assay has become a standard tool for scientists to study neural invasion by confronting the involved cell types. This protocol introduces Schwann cells, i.e., the most prevalent cell type in peripheral nerves, in a novel heterotypic, glia-cancer-neuron, 3D migration assay for assessing their relevance in the early pathogenesis of neural invasion. Particularly, this assay allows the monitoring of the early Schwann cell migratory activity.
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References
Ceyhan GO, Demir IE, Altintas B (2008) Neural invasion in pancreatic cancer: a mutual tropism between neurons and cancer cells. Biochem Biophys Res Commun 374:442–447
Demir IE, Boldis A, Pfitzinger PL (2014) Investigation of Schwann cells at neoplastic cell sites before the onset of cancer invasion. J Natl Cancer Inst 106:dju184
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Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G.O., Demir, I.E. (2018). 3D Cancer Migration Assay with Schwann Cells. In: Monje, P., Kim, H. (eds) Schwann Cells. Methods in Molecular Biology, vol 1739. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7649-2_20
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DOI: https://doi.org/10.1007/978-1-4939-7649-2_20
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7648-5
Online ISBN: 978-1-4939-7649-2
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