Abstract
Detection of human papilloma virus (HPV) in tissue specimens has been a clinical challenge since last 2 decades; however, screening for presence of E6/E7 transcripts is regarded as the gold standard, and it verifies the active HPV infection. Here, we describe “RNAscope® assay” a novel RNA in situ hybridization (ISH) technology; which detects E6/E7 mRNA of seven high risk HPV subtypes (HPV 16, 18, 31, 33, 35, 52, and 58) in formalin fixed paraffin embedded (FFPE) tissue samples.
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Pandey, M., Bhosale, P.G., Mahimkar, M.B. (2018). Detection of HPV E6/E7 mRNA in Clinical Samples Using RNA In Situ Hybridization. In: Santiago-Cardona, P. (eds) The Retinoblastoma Protein. Methods in Molecular Biology, vol 1726. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7565-5_15
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DOI: https://doi.org/10.1007/978-1-4939-7565-5_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7565-5
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