Abstract
Polymerase chain reaction enables the detection and characterization of circular RNA expression. The use of divergent primer pairs flanking the back-splice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.
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Acknowledgments
This work was supported by the German Cardiac Society (Deutsche Gesellschaft für Kardiologie (DGK)) to Jes-Niels Boeckel.
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Heumüller, A.W., Boeckel, JN. (2018). Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR. In: Dieterich, C., Papantonis, A. (eds) Circular RNAs. Methods in Molecular Biology, vol 1724. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7562-4_5
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DOI: https://doi.org/10.1007/978-1-4939-7562-4_5
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7562-4
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