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Labeling the ER for Light and Fluorescence Microscopy

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The Plant Endoplasmic Reticulum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1691))

Abstract

The ER is a highly dynamic network of tubules and membrane sheets. Hence imaging this organelle in its native and mobile state is of great importance. Here we describe methods of labeling the native ER using fluorescent proteins and lipid dyes as well as methods for immunolabeling on plant tissue.

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Author information

Authors and Affiliations

  1. Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK

    Chris Hawes & Verena Kriechbaumer

  2. Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, PR China

    Pengwei Wang

Authors
  1. Chris Hawes
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  2. Pengwei Wang
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  3. Verena Kriechbaumer
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Corresponding author

Correspondence to Chris Hawes .

Editor information

Editors and Affiliations

  1. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Chris Hawes

  2. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Verena Kriechbaumer

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Hawes, C., Wang, P., Kriechbaumer, V. (2018). Labeling the ER for Light and Fluorescence Microscopy. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7389-7_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7388-0

  • Online ISBN: 978-1-4939-7389-7

  • eBook Packages: Springer Protocols

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