The Plant Endoplasmic Reticulum

Methods and Protocols

  • Chris Hawes
  • Verena Kriechbaumer

Part of the Methods in Molecular Biology book series (MIMB, volume 1691)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Chris Hawes, Pengwei Wang, Verena Kriechbaumer
    Pages 1-14
  3. Maike Kittelmann
    Pages 15-21
  4. Pengwei Wang, Chris Hawes, Christine Richardson, Patrick J. Hussey
    Pages 23-31
  5. Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, Andreas J. Meyer
    Pages 43-66
  6. Natasha Dzimitrowicz, Emily Breeze, Lorenzo Frigerio
    Pages 67-74
  7. Verena Kriechbaumer
    Pages 117-123
  8. Laetitia Fouillen, Lilly Maneta-Peyret, Patrick Moreau
    Pages 125-137
  9. Dietmar G. Mehlhorn, Niklas Wallmeroth, Kenneth W. Berendzen, Christopher Grefen
    Pages 139-158
  10. Verena Kriechbaumer, Stanley W. Botchway
    Pages 159-166
  11. Imogen Sparkes, Rhiannon R. White, Benjamin Coles, Stanley W. Botchway, Andy Ward
    Pages 167-178
  12. Emanuela Pedrazzini, Alessandro Vitale
    Pages 179-189
  13. Lisa Yasmin Asseck, Niklas Wallmeroth, Christopher Grefen
    Pages 191-203
  14. Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit, Richard Strasser
    Pages 205-222
  15. Kazuki Tabara, Yuji Iwata, Nozomu Koizumi
    Pages 223-230
  16. Cristina Ruberti, Federica Brandizzi
    Pages 231-238

About this book

Introduction

This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Keywords

lipid analysis biosynthetic organelles super resolution imaging Auxin rafts

Editors and affiliations

  • Chris Hawes
    • 1
  • Verena Kriechbaumer
    • 2
  1. 1.Department of Biological and Medical SciencesOxford Brookes UniversityOxfordUnited Kingdom
  2. 2.Department of Biological and Medical SciencesOxford Brookes UniversityOxfordUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7389-7
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7388-0
  • Online ISBN 978-1-4939-7389-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book