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Rational and Semirational Protein Design

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Protein Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1685))

Abstract

This mini review gives an overview over different design approaches and methodologies applied in rational and semirational enzyme engineering. The underlying principles for engineering novel activities, enantioselectivity, substrate specificity, stability, and pH optimum are summarized.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY, 13244, USA

    Ivan V. Korendovych

Authors
  1. Ivan V. Korendovych
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Corresponding author

Correspondence to Ivan V. Korendovych .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry, Greifswald University, Greifswald, Germany

    Uwe T. Bornscheuer

  2. Protein Biochemistry Institute of Biochemistry, Greifswald University, Greifswald, Germany

    Matthias Höhne

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Korendovych, I.V. (2018). Rational and Semirational Protein Design. In: Bornscheuer, U., Höhne, M. (eds) Protein Engineering. Methods in Molecular Biology, vol 1685. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7366-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7366-8_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7364-4

  • Online ISBN: 978-1-4939-7366-8

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