Protein Engineering

Methods and Protocols

  • Uwe T. Bornscheuer
  • Matthias Höhne

Part of the Methods in Molecular Biology book series (MIMB, volume 1685)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Stefan Lutz, Samantha M. Iamurri
    Pages 1-12
  3. Computational Protocols

    1. Front Matter
      Pages 13-13
    2. Ivan V. Korendovych
      Pages 15-23
    3. Jan Brezovsky, Barbora Kozlikova, Jiri Damborsky
      Pages 25-42
    4. Henrik Land, Maria Svedendahl Humble
      Pages 43-67
    5. Hein J. Wijma, Maximilian J. L. J. Fürst, Dick B. Janssen
      Pages 69-85
    6. Carlos G. Acevedo-Rocha, Matteo Ferla, Manfred T. Reetz
      Pages 87-128
  4. Protein Library Expression

    1. Front Matter
      Pages 129-129
    2. Ana Luísa Ribeiro, Mario Mencía, Aurelio Hidalgo
      Pages 131-143
    3. Javier Santos-Aberturas, Mark Dörr, Uwe T. Bornscheuer
      Pages 157-170
    4. Srujan Kumar Dondapati, Doreen A. Wüstenhagen, Stefan Kubick
      Pages 171-186 Open Access
  5. Screening and Selection Assays

    1. Front Matter
      Pages 187-187
    2. Alexander Fulton, Marc R. Hayes, Ulrich Schwaneberg, Jörg Pietruszka, Karl-Erich Jaeger
      Pages 209-231
    3. Egon Heuson, Jean-Louis Petit, Franck Charmantray, Véronique de Bérardinis, Thierry Gefflaut
      Pages 233-245

About this book

Introduction

This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. 

Keywords

Protein tunnel inspection CAVER FRESCO algorithm split-GFP insect cell-free expression systems photometric screening solid phase agar

Editors and affiliations

  • Uwe T. Bornscheuer
    • 1
  • Matthias Höhne
    • 2
  1. 1.Department of Biotechnology and Enzyme Catalysis Institute of BiochemistryGreifswald UniversityGreifswaldGermany
  2. 2.Protein Biochemistry Institute of BiochemistryGreifswald UniversityGreifswaldGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7366-8
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7364-4
  • Online ISBN 978-1-4939-7366-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book