Protein Engineering
Methods and Protocols
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Part of the Methods in Molecular Biology book series (MIMB, volume 1685)
Table of contents
About this book
Introduction
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.
Keywords
Protein tunnel inspection CAVER FRESCO algorithm split-GFP insect cell-free expression systems photometric screening solid phase agar
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Bibliographic information
- DOI https://doi.org/10.1007/978-1-4939-7366-8
- Copyright Information Springer Science+Business Media LLC 2018
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-7364-4
- Online ISBN 978-1-4939-7366-8
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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