Abstract
Microencapsulation is a versatile method for enhancing the stability of bacteriophages under harsh conditions, such as those which occur during thermal processing. For food applications, encapsulation in food-grade polymer matrices is desirable owing to their nontoxicity and low cost. Here, we describe the encapsulation of Listeria phage A511 using sodium alginate, gum arabic, and gelatin to maximize its viability during thermal processing.
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References
Lim L-T (2015) Encapsulation of bioactive compounds using electrospinning and electrospraying technologies, Nanotechnology and functional foods. Wiley, Hoboken, NJ, pp 297–317
Hussain MA, Liu H, Wang Q, Zhong F, Guo Q, Balamurugan S (2017) Use of encapsulated bacteriophages to enhance farm to fork food safety. Crit Rev Food Sci Nutr 57:2801–2810
Kolanowski W, Laufenberg G, Kunz B (2004) Fish oil stabilisation by microencapsulation with modified cellulose. Int J Food Sci Nutr 55(4):333–343
Hategekimana J, Masamba KG, Ma J, Zhong F (2015) Encapsulation of vitamin E: effect of physicochemical properties of wall material on retention and stability. Carbohydr Polym 124:172–179
Chowdhuri S, Cole CM, Devaraj NK (2016) Encapsulation of living cells within giant phospholipid liposomes formed by the inverse-emulsion technique. Chembiochem 17(10):886–889
Zhou Y, Lim LT (2009) Activation of lactoperoxidase system in milk by glucose oxidase immobilized in electrospun polylactide microfibers. J Food Sci 74(2):C170–C176
Moomand K, Lim L-T (2015) Properties of encapsulated fish oil in electrospun zein fibres under simulated in vitro conditions. Food Bioprocess Technol 8(2):431–444
Kailasapathy K (2002) Microencapsulation of probiotic bacteria: technology and potential applications. Curr Issues Intest Microbiol 3(2):39–48
Gasperini L, Mano JF, Reis RL (2014) Natural polymers for the microencapsulation of cells. J R Soc Interface 11(100):20140817
Dembczynski R, Jankowski T (2002) Growth characteristics and acidifying activity of Lactobacillus rhamnosus in alginate/starch liquid-core capsules. Enzym Microb Technol 31(1–2):111–115
Gerez CL, Font de Valdez G, Gigante ML, Grosso CR (2012) Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamnosus CRL 1505 to low pH. Lett Appl Microbiol 54(6):552–556
Jiang T, Singh B, Maharjan S, Li H-S, Kang S-K, Bok J-D et al (2014) Oral delivery of probiotic expressing M cell homing peptide conjugated BmpB vaccine encapsulated into alginate/chitosan/alginate microcapsules. Eur J Pharm Biopharm 88(3):768–777
Lee KY, Heo TR (2000) Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. Appl Environ Microbiol 66(2):869–873
Ma Y, Pacan JC, Wang Q, Xu Y, Huang X, Korenevsky A et al (2008) Microencapsulation of bacteriophage felix O1 into chitosan-alginate microspheres for oral delivery. Appl Environ Microbiol 74(15):4799–4805
Annan NT, Borza AD, Hansen LT (2008) Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions. Food Res Int 41(2):184–193
Stanford K, McAllister TA, Niu YD, Stephens TP, Mazzocco A, Waddell TE et al (2010) Oral delivery systems for encapsulated bacteriophages targeted at Escherichia coli O157:H7 in feedlot cattle. J Food Prot 73(7):1304–1312
Tang Z, Huang X, Baxi S, Chambers JR, Sabour PM, Wang Q (2013) Whey protein improves survival and release characteristics of bacteriophage Felix O1 encapsulated in alginate microspheres. Food Res Int 52(2):460–466
Speaker TJ, Clark HF, Moser CA, Offit PA, Campos M, Frenchick PJ (2001) Aqueous solvent based encapsulation of a bovine herpes virus type-1 subunit vaccine. Patent EP0873752B1, European Patent register.
Korehei R, Kadla JF (2014) Encapsulation of T4 bacteriophage in electrospun poly(ethylene oxide)/cellulose diacetate fibers. Carbohydr Polym 100:150–157
Kropinski AM, Mazzocco A, Waddell TE, Lingohr E, Johnson RP (2009) Enumeration of bacteriophages by double agar overlay plaque assay. Methods Mol Biol 501:69–76
Radford DR, Ahmadi H, Leon-Velarde CG, Balamurugan S (2016) Propagation method for persistent high yield of diverse Listeria phages on permissive hosts at refrigeration temperatures. Res Microbiol 167(8):685–691
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This work was supported by Agriculture and Agri-Food Canada.
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Ahmadi, H., Wang, Q., Lim, LT., Balamurugan, S. (2018). Encapsulation of Listeria Phage A511 by Alginate to Improve Its Thermal Stability. In: Clokie, M., Kropinski, A., Lavigne, R. (eds) Bacteriophages. Methods in Molecular Biology, vol 1681. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7343-9_7
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DOI: https://doi.org/10.1007/978-1-4939-7343-9_7
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