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Detection of DNA-RNA Hybrids In Vivo

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Genome Instability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1672))

Abstract

DNA-RNA hybrids form naturally during essential cellular functions such as transcription and replication. However, they may be an important source of genome instability, a hallmark of cancer and genetic diseases. Detection of DNA-RNA hybrids in cells is becoming crucial to understand an increasing number of molecular biology processes in genome dynamics and function and to identify new factors and mechanisms responsible for disease in biomedical research. Here, we describe two different procedures for the reliable detection of DNA-RNA hybrids in the yeast Saccharomyces cerevisiae and in human cells: DNA-RNA Immunoprecipitation (DRIP) and Immunofluorescence.

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Acknowledgments

A.A.’s lab is funded by Spanish Ministry of Economy and Competitiveness, Junta de Andalucía, European Research Council, the Worldwide Cancer Research and the European Union (FEDER).

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Author notes

  1. María García-Rubio and Sonia I. Barroso contributed equally to this work.

Authors and Affiliations

  1. Centro Andaluz de Biología Molecular y Medicina Regenerativa, CABIMER, Universidad de Sevilla, Av. Américo Vespucio 24, 41092, Seville, Spain

    María García-Rubio, Sonia I. Barroso & Andrés Aguilera

Authors
  1. María García-Rubio
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  2. Sonia I. Barroso
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  3. Andrés Aguilera
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Corresponding author

Correspondence to Andrés Aguilera .

Editor information

Editors and Affiliations

  1. Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Milano, Italy

    Marco Muzi-Falconi

  2. Donnelly Centre and Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

    Grant W Brown

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García-Rubio, M., Barroso, S.I., Aguilera, A. (2018). Detection of DNA-RNA Hybrids In Vivo. In: Muzi-Falconi, M., Brown, G. (eds) Genome Instability. Methods in Molecular Biology, vol 1672. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7306-4_24

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  • DOI: https://doi.org/10.1007/978-1-4939-7306-4_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7305-7

  • Online ISBN: 978-1-4939-7306-4

  • eBook Packages: Springer Protocols

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