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Isolation of Extracellular Vesicles by Ultracentrifugation

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Extracellular Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Extracellular vesicles (EVs) represent a group of heterogeneous vesicles that can be obtained from almost all biofluids. EVs, including microvesicles, exosomes, and apoptotic bodies , can deliver bioactive cargos and signaling molecules. Various physiological roles and pathophysiological roles for EVs in diseases such as cancer, infectious diseases, endocrine diseases, and neurodegenerative disorders have been recognized. These observations highlight EVs as potential novel biomarkers and targets for therapeutic intervention. One of the major limitations in the use of EVs for diagnosis and therapeutic purposes is the lack of standardization of isolation techniques. Here, we describe protocols for ultracentrifugation and sucrose gradient isolation methods, which are the current gold standard, and are the most studied methods for EV isolation.

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Author information

Authors and Affiliations

  1. Columbia College of Dental Medicine, 622 W 168th St, New York, NY, 10032, USA

    Fatemeh Momen-Heravi DDS, PhD, MPH

Authors
  1. Fatemeh Momen-Heravi DDS, PhD, MPH
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Correspondence to Fatemeh Momen-Heravi DDS, PhD, MPH .

Editor information

Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Momen-Heravi, F. (2017). Isolation of Extracellular Vesicles by Ultracentrifugation. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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