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Characterization of Extracellular Vesicles by Surface Plasmon Resonance

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Extracellular Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Surface plasmon resonance (SPR ) enables real-time, label-free detection of ligand binding to target receptors immobilized on a sensing surface. SPR has emerged as a promising technique for extracellular vesicle (EV) characterization with its label-free detection scheme, and exquisite sensitivity. Among the various system configurations, nanohole-based SPR sensors are of particular interest because of their simple optical setup, tunability, and scalability. Here, we describe the characterization of circulating EVs or exosomes from human clinical samples using a nanohole-based SPR sensor, named nPLEX (nano-plasmonic exosome) sensing.

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Acknowledgment

This work was supported in part by US NIH Grants R01-HL113156 (to H.L.), K12CA087723-11 A1 (to C.M.C.) and 1K99CA201248-01 (to H.I.), the Massachusetts General Hospital Physician Scientist Development Award (to C.M.C.), and the Department of Defense Ovarian Cancer Research Program Award W81XWH-14-1-0279 (to H.L.)

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Authors and Affiliations

  1. Cancer Program, MGH Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

    Hyungsoon Im, Katherine Yang, Hakho Lee & Cesar M. Castro

  2. Division of Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA, 02114, USA

    Cesar M. Castro

Authors
  1. Hyungsoon Im
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  2. Katherine Yang
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  3. Hakho Lee
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  4. Cesar M. Castro
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Corresponding author

Correspondence to Cesar M. Castro .

Editor information

Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Im, H., Yang, K., Lee, H., Castro, C.M. (2017). Characterization of Extracellular Vesicles by Surface Plasmon Resonance. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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