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Solid Phase Immunoassay for the Detection of Anticardiolipin Antibodies

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Hemostasis and Thrombosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1646))

Abstract

The anticardiolipin (aCL) test was first developed in the 1980s and proved to be a valuable addition to the lupus anticoagulant assay for identifying patients with a disorder that came to be later known as the antiphospholipid syndrome (APS). Although the test has relatively poor specificity for APS diagnosis, particularly at low positive levels, it has continued to play a major role in the identification and management of these patients because of its high sensitivity and ability to be measured in both serum and plasma, and despite concomitant presence of anticoagulants normally given to APS patients. In this chapter we outline the procedure for producing essential assay components and for performing the aCL ELISA, which can be used to determine the presence of IgG, IgM and IgA aCL antibodies in human samples. We also provide general guidelines that will facilitate optimal performance of the aCL ELISA assay.

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Acknowledgement

RW is supported by a Mallinckrodt-Questcor Fellowship Grant and both RW and EP are supported by an NIH Grant R01 AR056745-01A1.

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Authors and Affiliations

  1. Antiphospholipid Standardization Laboratory, Division of Rheumatology, Department of Internal Medicine, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, 77555-0883, USA

    Rohan Willis & Elizabeth Papalardo

  2. University of the West Indies, Mona Campus, Kingston, Jamaica, WI

    E. Nigel Harris

Authors
  1. Rohan Willis
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  2. Elizabeth Papalardo
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  3. E. Nigel Harris
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Corresponding author

Correspondence to Rohan Willis .

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Editors and Affiliations

  1. Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, New South Wales, Australia

    Emmanuel J. Favaloro

  2. Section of Clinical Biochemistry, University of Verona, Verona, Italy

    Giuseppe Lippi

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Willis, R., Papalardo, E., Nigel Harris, E. (2017). Solid Phase Immunoassay for the Detection of Anticardiolipin Antibodies. In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7196-1_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7194-7

  • Online ISBN: 978-1-4939-7196-1

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