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Genome Editing of Silkworms

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Genome Editing in Animals

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1630))

Abstract

Silkworm is a lepidopteran insect that has been used as a model for a wide variety of biological studies. The microinjection technique is available and it is possible to cause transgenesis as well as target gene disruption via the genome editing technique. TALEN-mediated knock-out is especially effective in this species. We also succeeded in the precise and efficient integration of a donor vector using the Precise Integration into Target Chromosome (PITCh) method. Here, we describe protocols for ZFN, TALEN, and CRISPR/Cas9-mediated genome editing as well as the PITCh technique in the silkworm. We consider that all of these techniques can contribute to the further promotion of various biological studies in the silkworm and other insect species.

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Acknowledgment

This work was supported by grants-in-aid from the Ministry of Agriculture, Forestry, and Fisheries.

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Authors and Affiliations

  1. Transgenic Silkworm Research Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan

    Takuya Tsubota & Hideki Sezutsu

Authors
  1. Takuya Tsubota
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  2. Hideki Sezutsu
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Corresponding author

Correspondence to Hideki Sezutsu .

Editor information

Editors and Affiliations

  1. Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

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Tsubota, T., Sezutsu, H. (2017). Genome Editing of Silkworms. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7128-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7127-5

  • Online ISBN: 978-1-4939-7128-2

  • eBook Packages: Springer Protocols

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