Skip to main content
SpringerLink
Log in

Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools

  • Chapter
  • First Online:
Targeted Genome Editing Using Site-Specific Nucleases

  • 3914 Accesses

Abstract

The silkworm, Bombyx mori, is a classic model organism in studies of insect genetics and physiology. As B. mori and most other lepidopteran species (moths and butterflies) are generally refractory to RNA interference (RNAi), it has been very difficult to conduct loss-of-function studies of genes in lepidopterans. However, recent advances in genome engineering tools, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced palindromic repeats/CRISPR-associated (CRISPR/Cas) system, have dramatically changed this situation. Although efficiency of targeted mutagenesis in B. mori was very low in an early experiment using ZFNs, recent studies using TALENs and CRISPR/Cas9 have induced highly efficient mutagenesis of the target genes in B. mori, even when the mutant phenotype was unknown. Genome editing tools facilitate sophisticated genetic manipulation and breeding of a wide variety of both beneficial and pest insects. This chapter summarizes recent advances in genome editing techniques in B. mori and proposes guidelines for experimental design and strategy for successful gene knockout experiments using this species.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Ando T, Fujiwara H (2012) Electroporation-mediated somatic transgenesis for rapid functional analysis in insects. Development 140:454–458

    Article  Google Scholar 

  • Banno Y, Fujii H, Kawaguchi Y, Yamamoto K, Nishikawa K, Nishisaka A, Tamura K, Eguchi S (2005) A guide to the silkworm mutants 2005-gene name and gene. Institute of Genetic Resorces, Kyushu University, Fukuoka, Japan, Silkworm Genetics Division

    Google Scholar 

  • Bassett AR, Tibbit C, Ponting CP, Liu JL (2013) Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell Rep 4:220–228

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Beech CJ, Koukidou M, Morrison NI, Alphey L (2012) Genetically modified insects: science, use, status and regulation. Collection Biosafety Rev 6:66–124

    Google Scholar 

  • Beumer KJ, Trautman JK, Bozas A, Liu JL, Rutter J, Gall JG, Carroll D (2008) Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc Natl Acad Sci U S A 105:19821–19826

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Boch J, Scholze H, Schornack S, Landgraf A, Hahn S, Kay S, Lahaye T, Nickstadt A, Bonas U (2009) Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326:1509–1512

    Article  CAS  PubMed  Google Scholar 

  • Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF (2011) Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 39:e82

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B (2013) Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 155:1479–1491

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cho SW, Kim S, Kim JM, Kim JS (2013) Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol 31:230–232

    Article  CAS  PubMed  Google Scholar 

  • Christian M, Cermak T, Doyle EL, Schmidt C, Zhang F, Hummel A, Bogdanove AJ, Voytas DF (2010) Targeting DNA double-strand breaks with TAL effector nucleases. Genetics 186:757–761

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F (2012) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun 3:968

    Article  PubMed Central  PubMed  Google Scholar 

  • Crocker J, Stern DL (2013) TALE-mediated modulation of transcriptional enhancers in vivo. Nat Methods 10:762–767

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Daimon T, Kiuchi T, Takasu Y (2014) Recent progress in genome engineering techniques in the silkworm, Bombyx mori. Dev Growth Differ 56:14–25

    Article  CAS  PubMed  Google Scholar 

  • Doyle EL, Booher NJ, Standage DS, Voytas DF, Brendel VP, Vandyk JK, Bogdanove AJ (2012) TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction. Nucleic Acids Res 40 (Web Server issue):W117–122

    Google Scholar 

  • Duan J, Xu H, Ma S, Guo H, Wang F, Zhao P, Xia Q (2013) Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori). Transgenic Res 22:607–619

    Article  CAS  PubMed  Google Scholar 

  • Ferguson HJ, Neven LG, Thibault ST, Mohammed A, Fraser M (2011) Genetic transformation of the codling moth, Cydia pomonella L., with piggyBac EGFP. Transgenic Res 20:201–214

    Article  CAS  PubMed  Google Scholar 

  • Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD (2013) High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol 31:822–826

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Goldsmith MR, Shimada T, Abe H (2005) The genetics and genomics of the silkworm, Bombyx mori. Annu Rev Entomol 50:71–100

    Article  CAS  PubMed  Google Scholar 

  • Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM (2013) Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics 194:1029–1035

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Grau J, Boch J, Posch S (2013) TALENoffer: genome-wide TALEN off-target prediction. Bioinformatics 29:2931

    Article  CAS  PubMed  Google Scholar 

  • Handler AM (2002) Use of the piggyBac transposon for germ-line transformation of insects. Insect Biochem Mol Biol 32:1211–1220

    Article  CAS  PubMed  Google Scholar 

  • Handler AM, McCombs SD (2000) The piggyBac transposon mediates germ-line transformation in the oriental fruit fly and closely related elements exist in its genome. Insect Mol Biol 9:605–612

    Article  CAS  PubMed  Google Scholar 

  • Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q et al (2011) Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol 29:731–734

    Google Scholar 

  • Hwang WY, Fu Y, Reyon D, Maeder ML, Kaini P, Sander JD, Joung JK, Peterson RT, Yeh JR (2013a) Heritable and precise zebrafish genome editing using a CRISPR-Cas system. PLoS One 8:e68708

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK (2013b) Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol 31:227–229

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Imamura M, Nakai J, Inoue S, Quan GX, Kanda T, Tamura T (2003) Targeted gene expression using the GAL4/UAS system in the silkworm Bombyx mori. Genetics 165:1329–1340

    CAS  PubMed Central  PubMed  Google Scholar 

  • Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA (2013) RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31:233–239

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Joung JK, Sander JD (2013) TALENs: a widely applicable technology for targeted genome editing. Nat Rev Mol Cell Biol 14:49–55

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kamimura M, Saito H, Niwa R, Niimi T, Toyoda K, Ueno C, Kanamori Y, Shimura S, Kiuchi M (2012) Fungal ecdysteroid-22-oxidase, a new tool for manipulating ecdysteroid signaling and insect development. J Biol Chem 287:16488–16498

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kobayashi I, Kojima K, Uchino K, Sezutsu H, Iizuka T, Tatematsu KI, Yonemura N, Tanaka H, Yamakawa M, Ogura E, Kamachi Y, Tamura T (2011) An efficient binary system for gene expression in the silkworm, Bombyx mori, using Gal4 variants. Arch Insect Biochem Physiol 76:195–210

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi I, Tsukioka H, Komoto N, Uchino K, Sezutsu H, Tamura T, Kusakabe T, Tomita S (2012) SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells. Insect Biochem Mol Biol 42:148–154

    Article  CAS  PubMed  Google Scholar 

  • Kokoza V, Ahmed A, Wimmer EA, Raikhel AS (2001) Efficient transformation of the yellow fever mosquito Aedes aegypti using the piggyBac transposable element vector pBac[3xP3-EGFP afm]. Insect Biochem Mol Biol 31:1137–1143

    Article  CAS  PubMed  Google Scholar 

  • Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le C, Platt RJ, Scott DA, Church GM, Zhang F (2013) Optical control of mammalian endogenous transcription and epigenetic states. Nature 500:472–476

    CAS  PubMed  Google Scholar 

  • Langmead B, Trapnell C, Pop M, Salzberg SL (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10:R25

    Article  PubMed Central  PubMed  Google Scholar 

  • Liu D, Yan S, Huang Y, Tan A, Stanley DW, Song Q (2012a) Genetic transformation mediated by piggyBac in the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Arch Insect Biochem Physiol 80:140–150

    Article  CAS  PubMed  Google Scholar 

  • Liu J, Li C, Yu Z, Huang P, Wu H, Wei C, Zhu N, Shen Y, Chen Y, Zhang B, Deng WM, Jiao R (2012b) Efficient and specific modifications of the Drosophila genome by means of an easy TALEN strategy. J Genet Genomics 39:209–215

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Ma S, Wang X, Chang J, Gao J, Shi R, Zhang J, Lu W, Zhao P, Xia Q (2014) Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9. Insect Biochem Mol Biol 49:35–42

    Article  CAS  PubMed  Google Scholar 

  • Lorenzen MD, Berghammer AJ, Brown SJ, Denell RE, Klingler M, Beeman RW (2003) piggyBac-mediated germline transformation in the beetle Tribolium castaneum. Insect Mol Biol 12:433–440

    Article  CAS  PubMed  Google Scholar 

  • Ma S, Zhang S, Wang F, Liu Y, Xu H, Liu C, Lin Y, Zhao P, Xia Q (2012) Highly efficient and specific genome editing in silkworm using custom TALENs. PLoS One 7:e45035

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ma S, Chang J, Wang X, Liu Y, Zhang J, Lu W, Gao J, Shi R, Zhao P, Xia Q (2014a) CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori. Sci Rep 4:4489

    PubMed Central  PubMed  Google Scholar 

  • Ma S, Wang X, Liu Y, Gao J, Zhang S, Shi R, Chang J, Zhao P, Xia Q (2014b) Multiplex genomic structure variation mediated by TALEN and ssODN. BMC Genomics 15:41

    Article  PubMed Central  PubMed  Google Scholar 

  • Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM (2013) CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol 31:833–838

    Article  CAS  PubMed  Google Scholar 

  • Marcus JM, Ramos DM, Monteiro A (2004) Germline transformation of the butterfly Bicyclus anynana. Proc Biol Sci 271(Suppl 5):S263–S265

    Article  PubMed Central  PubMed  Google Scholar 

  • Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, Dafa'alla T, Alphey L (2012) Germline transformation of the diamondback moth, Plutella xylostella L., using the piggyBac transposable element. Insect Mol Biol 21:414–421

    Article  CAS  PubMed  Google Scholar 

  • Merlin C, Beaver LE, Taylor OR, Wolfe SA, Reppert SM (2013) Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases. Genome Res 23:159–168

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Miller JC, Tan S, Qiao G, Barlow KA, Wang J, Xia DF, Meng X, Paschon DE, Leung E, Hinkley SJ, Dulay GP, Hua KL, Ankoudinova I, Cost GJ, Urnov FD, Zhang HS, Holmes MC, Zhang L, Gregory PD, Rebar EJ (2011) A TALE nuclease architecture for efficient genome editing. Nat Biotechnol 29:143–148

    Article  CAS  PubMed  Google Scholar 

  • Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin IT, Abe H, Shimada T, Morishita S, Sasaki T (2004) The genome sequence of silkworm, Bombyx mori. DNA Res 11:27–35

    Article  CAS  PubMed  Google Scholar 

  • Miyanari Y, Ziegler-Birling C, Torres-Padilla ME (2013) Live visualization of chromatin dynamics with fluorescent TALEs. Nat Struct Mol Biol 20:1321–1324

    Article  CAS  PubMed  Google Scholar 

  • Moscou MJ, Bogdanove AJ (2009) A simple cipher governs DNA recognition by TAL effectors. Science 326:1501

    Article  CAS  PubMed  Google Scholar 

  • Nakamura T, Yoshizaki M, Ogawa S, Okamoto H, Shinmyo Y, Bando T, Ohuchi H, Noji S, Mito T (2010) Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism. Curr Biol 20:1641–1647

    Article  CAS  PubMed  Google Scholar 

  • Pattanayak V, Ramirez CL, Joung JK, Liu DR (2011) Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat Methods 8:765–770

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Peloquin JJ, Thibault ST, Staten R, Miller TA (2000) Germ-line transformation of pink bollworm (Lepidoptera: gelechiidae) mediated by the piggyBac transposable element. Insect Mol Biol 9:323–333

    Article  CAS  PubMed  Google Scholar 

  • Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152:1173–1183

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Sajwan S, Takasu Y, Tamura T, Uchino K, Sezutsu H, Zurovec M (2013) Efficient disruption of endogenous Bombyx gene by TAL effector nucleases. Insect Biochem Mol Biol 43:17–23

    Article  CAS  PubMed  Google Scholar 

  • Sakuma T, Woltjen K (2014) Nuclease-mediated genome editing: at the front-line of functional genomics technology. Dev Growth Differ 56:2–13

    Article  CAS  PubMed  Google Scholar 

  • Sakuma T, Hosoi S, Woltjen K, Suzuki K, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T (2013) Efficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells 18:315–326

    Article  CAS  PubMed  Google Scholar 

  • Subbaiah EV, Royer C, Kanginakudru S, Satyavathi VV, Babu AS, Sivaprasad V, Chavancy G, Darocha M, Jalabert A, Mauchamp B, Basha I, Couble P, Nagaraju J (2013) Engineering silkworms for resistance to baculovirus through multigene RNA interference. Genetics 193:63–75

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Sumitani M, Yamamoto DS, Oishi K, Lee JM, Hatakeyama M (2003) Germline transformation of the sawfly, Athalia rosae (Hymenoptera: Symphyta), mediated by a piggyBac-derived vector. Insect Biochem Mol Biol 33:449–458

    Article  CAS  PubMed  Google Scholar 

  • Takasu Y, Kobayashi I, Beumer K, Uchino K, Sezutsu H, Sajwan S, Carroll D, Tamura T, Zurovec M (2010) Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection. Insect Biochem Mol Biol 40:759–765

    Article  CAS  PubMed  Google Scholar 

  • Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu H, Tamura T, Zurovec M (2013) Efficient TALEN construction for Bombyx gene targeting. PLoS One 18:e73458

    Article  Google Scholar 

  • Tamura T, Thibert C, Royer C, Kanda T, Abraham E, Kamba M, Komoto N, Thomas JL, Mauchamp B, Chavancy G, Shirk P, Fraser M, Prudhomme JC, Couble P (2000) Germline transformation of the silkworm Bombyx mori L. Using a piggyBac transposon-derived vector. Nat Biotechnol 18:81–84

    Article  CAS  PubMed  Google Scholar 

  • Tazima Y (1964) The genetics of the silkworm. Logos Press, London

    Google Scholar 

  • Tazima Y (1978) The silkworm: an important tool. Kodansha, Tokyo

    Google Scholar 

  • Terenius O, Papanicolaou A, Garbutt JS, Eleftherianos I, Huvenne H, Kanginakudru S, Albrechtsen M, An C, Aymeric JL, Barthel A, Bebas P, Bitra K, Bravo A, Chevalier F, Collinge DP, Crava CM, de Maagd RA, Duvic B, Erlandson M, Faye I, Felfoldi G, Fujiwara H, Futahashi R, Gandhe AS, Gatehouse HS, Gatehouse LN, Giebultowicz JM, Gomez I, Grimmelikhuijzen CJ, Groot AT, Hauser F, Heckel DG, Hegedus DD, Hrycaj S, Huang L, Hull JJ, Iatrou K, Iga M, Kanost MR, Kotwica J, Li C, Li J, Liu J, Lundmark M, Matsumoto S, Meyering-Vos M, Millichap PJ, Monteiro A, Mrinal N, Niimi T, Nowara D, Ohnishi A, Oostra V, Ozaki K, Papakonstantinou M, Popadic A, Rajam MV, Saenko S, Simpson RM, Soberon M, Strand MR, Tomita S, Toprak U, Wang P, Wee CW, Whyard S, Zhang W, Nagaraju J, Ffrench-Constant RH, Herrero S, Gordon K, Swevers L, Smagghe G (2011) RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design. J Insect Physiol 57:231–245

    Article  CAS  PubMed  Google Scholar 

  • The International Silkworm Genome Consortium (2008) The genome of a lepidopteran model insect, the silkworm Bombyx mori. Insect Biochem Mol Biol 38:1036–1045

    Article  CAS  Google Scholar 

  • Tomita M, Munetsuna H, Sato T, Adachi T, Hino R, Hayashi M, Shimizu K, Nakamura N, Tamura T, Yoshizato K (2003) Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat Biotechnol 21:52–56

    Article  CAS  PubMed  Google Scholar 

  • Uchino K, Imamura M, Shimizu K, Kanda T, Tamura T (2007) Germ line transformation of the silkworm, Bombyx mori, using the transposable element Minos. Mol Genet Genomics 277:213–220

    Article  CAS  PubMed  Google Scholar 

  • Uchino K, Sezutsu H, Imamura M, Kobayashi I, Tatematsu K, Iizuka T, Yonemura N, Mita K, Tamura T (2008) Construction of a piggyBac-based enhancer trap system for the analysis of gene function in silkworm Bombyx mori. Insect Biochem Mol Biol 38:1165–1173

    Article  CAS  PubMed  Google Scholar 

  • Uhlirova M, Foy BD, Beaty BJ, Olson KE, Riddiford LM, Jindra M (2003) Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of broad-complex in insect metamorphosis. Proc Natl Acad Sci U S A 100:15607–15612

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Urnov FD, Rebar EJ, Holmes MC, Zhang HS, Gregory PD (2010) Genome editing with engineered zinc finger nucleases. Nat Rev Genet 11:636–646

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Li Z, Xu J, Zeng B, Ling L, You L, Chen Y, Huang Y, Tan A (2013) The CRISPR/Cas system mediates efficient genome engineering in Bombyx mori. Cell Res 23:1414–1416

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T (2012) Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases. Nat Commun 3:1017

    Article  PubMed Central  PubMed  Google Scholar 

  • Xia Q, Zhou Z, Lu C, Cheng D, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, Wan Y, Zhu Y, Yu M, Shen W, Wu D, Xiang Z, Yu J, Wang J, Li R, Shi J, Li H, Su J, Wang X, Zhang Z, Wu Q, Li J, Zhang Q, Wei N, Sun H, Dong L, Liu D, Zhao S, Zhao X, Meng Q, Lan F, Huang X, Li Y, Fang L, Li D, Sun Y, Yang Z, Huang Y, Xi Y, Qi Q, He D, Huang H, Zhang X, Wang Z, Li W, Cao Y, Yu Y, Yu H, Ye J, Chen H, Zhou Y, Liu B, Ji H, Li S, Ni P, Zhang J, Zhang Y, Zheng H, Mao B, Wang W, Ye C, Wong GK, Yang H (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306:1937–1940

    Article  PubMed  Google Scholar 

  • Xiao A, Wang Z, Hu Y, Wu Y, Luo Z, Yang Z, Zu Y, Li W, Huang P, Tong X, Zhu Z, Lin S, Zhang B (2013a) Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish. Nucleic Acids Res 41:e141

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xiao A, Wu Y, Yang Z, Hu Y, Wang W, Zhang Y, Kong L, Gao G, Zhu Z, Lin S, Zhang B (2013b) EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineering. Nucleic Acids Res 41(Database issue):D415–D422

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yamaguchi J, Mizoguchi T, Fujiwara H (2011) siRNAs induce efficient RNAi response in Bombyx mori embryos. PLoS One 6:e25469

    Google Scholar 

  • Yu Z, Ren M, Wang Z, Zhang B, Rong YS, Jiao R, Gao G (2013) Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila. Genetics 195:289–291

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba city, Ibaraki, 305-8634, Japan

    Takaaki Daimon

Authors
  1. Takaaki Daimon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takaaki Daimon .

Editor information

Editors and Affiliations

  1. Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan

    Takashi Yamamoto

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Japan

About this chapter

Cite this chapter

Daimon, T. (2015). Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools. In: Yamamoto, T. (eds) Targeted Genome Editing Using Site-Specific Nucleases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55227-7_5

Download citation

Publish with us

Policies and ethics

Search

Navigation