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Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa

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Cryopreservation of Mammalian Gametes and Embryos

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

Abstract

The aim of this chapter was to describe the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment.

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Authors and Affiliations

  1. Department of Obsterics and Gynecology, Medical Faculty, University Maternal Hospital, Cologne University, Kerpener Str 34, 50931, Cologne, Germany

    Vladimir Isachenko, Peter Mallmann, Gohar Rahimi & Evgenia Isachenko

  2. Department of Preclinical Science, Facultad de Medicina, Center for Traslation Medicine, Universidad de la Frontera, Avenida Alemania, 0458, Temuco, Chile

    Raul Sanchez

Authors
  1. Vladimir Isachenko
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  2. Raul Sanchez
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  3. Peter Mallmann
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  4. Gohar Rahimi
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  5. Evgenia Isachenko
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Corresponding author

Correspondence to Vladimir Isachenko .

Editor information

Editors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia, USA

    Zsolt Peter Nagy

  2. ASTRA Fertility Group, Mississauga, Ontario, Canada

    Alex C. Varghese

  3. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Ashok Agarwal

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Isachenko, V., Sanchez, R., Mallmann, P., Rahimi, G., Isachenko, E. (2017). Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

  • eBook Packages: Springer Protocols

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