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Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos

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Morpholino Oligomers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1565))

Abstract

Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.

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Acknowledgments

This study was supported in part by NIH grant HL68854.

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Authors and Affiliations

  1. Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA

    Qiuming Gong & Zhengfeng Zhou

Authors
  1. Qiuming Gong
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  2. Zhengfeng Zhou
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Corresponding author

Correspondence to Zhengfeng Zhou .

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Editors and Affiliations

  1. Department of Biomedical Sciences College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, USA

    Hong M. Moulton

  2. Gene Tools, LLC, Philomath, Oregon, USA

    Jon D. Moulton

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Gong, Q., Zhou, Z. (2017). Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos. In: Moulton, H., Moulton, J. (eds) Morpholino Oligomers. Methods in Molecular Biology, vol 1565. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6817-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6817-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6815-2

  • Online ISBN: 978-1-4939-6817-6

  • eBook Packages: Springer Protocols

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