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Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray

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Mycotoxigenic Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1542))

Abstract

Various bioanalytical tools including DNA microarrays are frequently used to map global transcriptional changes in mycotoxin producer filamentous fungi. This effective hybridization-based transcriptomics technology helps researchers to identify genes of secondary metabolite gene clusters and record concomitant gene expression changes in these clusters initiated by versatile environmental conditions and/or gene deletions. Such transcriptional data are of great value when future mycotoxin control technologies are considered and elaborated. Giving the readers insights into RNA extraction and DNA microarray hybridization steps routinely used in our laboratories and also into the normalization and evaluation of primary gene expression data, we would like to contribute to the interlaboratory standardization of DNA microarray based transcriptomics studies being carried out in many laboratories worldwide in this important field of fungal biology.

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Acknowledgements

This work was supported by the Hungarian Scientific Research Fund (OTKA K100464, K112181) and by the SROP-4.2.2.B-15/1/KONV-2015-0001 project. The project has been supported by the European Union, cofinanced by the European Social Fund.

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Authors and Affiliations

  1. Faculty of Science and Technology, Department of Biotechnology and Microbiology,, University of Debrecen, Egyetem tér 1., H-4032, Debrecen, Hungary

    Tamás Emri & István Pócsi

  2. Kromat Ltd., Péterhegyi út 98., H-1112, Budapest, Hungary

    Anna Zalka

Authors
  1. Tamás Emri
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  2. Anna Zalka
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  3. István Pócsi
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Corresponding author

Correspondence to István Pócsi .

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Editors and Affiliations

  1. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonio Moretti

  2. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonia Susca

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Emri, T., Zalka, A., Pócsi, I. (2017). Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray. In: Moretti, A., Susca, A. (eds) Mycotoxigenic Fungi. Methods in Molecular Biology, vol 1542. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6707-0_23

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  • DOI: https://doi.org/10.1007/978-1-4939-6707-0_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6705-6

  • Online ISBN: 978-1-4939-6707-0

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