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Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

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Cell Cycle Synchronization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1524))

Abstract

The efficient use of hematopoietic stem cells (HSC) for transplantation is often limited by the relatively low numbers of HSC collected. The ex vivo expansion of HSC for clinical use is a potentially valuable and safe approach to increase HSC numbers thereby increasing engraftment and reducing the risk of morbidity from infection. Here, we describe a protocol for the robust ex vivo expansion of human CD34(+) HSC isolated from umbilical cord blood. The protocol described can efficiently generate large numbers of HSC. We also describe a flow cytometry-based method using high-resolution division tracking to characterize the kinetics of HSC growth and differentiation. Utilizing the guidelines discussed, it is possible for investigators to use this protocol as presented or to modify it for their specific needs.

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Acknowledgements

The authors thank the Australian Cord Blood Bank for providing cord blood.

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Authors and Affiliations

  1. Sydney Cord and Marrow Transplant Facility, Sydney Children’s Hospital, Graduate School of Biomedical Engineering, University of New South Wales, High Street, Randwick, NSW, 2031, Australia

    Kap-Hyoun Ko & Alla Dolnikov

  2. Graduate School of Biomedical Engineering, University of New South Wales, Kensington, NSW, Australia

    Robert Nordon

  3. Sydney Cord and Marrow Transplant Facility, Sydney Children’s Hospital, Centre for Children’s Cancer and Blood Disorders, Sydney Children’s Hospital, Randwick, NSW, Australia

    Tracey A. O’Brien

  4. Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia

    Geoff Symonds & Alla Dolnikov

Authors
  1. Kap-Hyoun Ko
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  2. Robert Nordon
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  3. Tracey A. O’Brien
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  4. Geoff Symonds
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  5. Alla Dolnikov
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Corresponding author

Correspondence to Alla Dolnikov .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary

    Gaspar Banfalvi

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Ko, KH., Nordon, R., O’Brien, T.A., Symonds, G., Dolnikov, A. (2017). Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation. In: Banfalvi, G. (eds) Cell Cycle Synchronization. Methods in Molecular Biology, vol 1524. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6603-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6603-5_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6602-8

  • Online ISBN: 978-1-4939-6603-5

  • eBook Packages: Springer Protocols

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