Cell Cycle Synchronization

Methods and Protocols

  • Gaspar Banfalvi
Part of the Methods in Molecular Biology book series (MIMB, volume 1524)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Cell Cycle Synchronization

    1. Front Matter
      Pages 1-1
    2. Gaspar Banfalvi
      Pages 3-27
  3. Physical Fractionation

    1. Front Matter
      Pages 29-29
    2. Laura Senovilla, Yohann Demont, Juliette Humeau, Norma Bloy, Guido Kroemer
      Pages 53-64
    3. Kalyan Dulla, Anna Santamaria Margalef
      Pages 65-74
  4. Chemical Blockade

    1. Front Matter
      Pages 75-75
    2. Thomas J. Langan, Kyla R. Rodgers, Richard C. Chou
      Pages 97-105
    3. Dorota Halicka, Hong Zhao, Jiangwei Li, Jorge Garcia, Monika Podhorecka, Zbigniew Darzynkiewicz
      Pages 107-119
    4. Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas, Gaspar Banfalvi
      Pages 161-176
    5. Jennifer A. Fischer, Salvatore J. Caradonna
      Pages 177-188
    6. Hoi Tang Ma, Randy Y. C. Poon
      Pages 189-201
  5. Synchronization of Unicellular Organisms

    1. Front Matter
      Pages 203-203
    2. Jessica Smith, Arkadi Manukyan, Hui Hua, Huzefa Dungrawala, Brandt L. Schneider
      Pages 215-242
    3. Staffan Svärd, Karin Troell
      Pages 243-252

About this book

Introduction

This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.

Keywords

Unicellular organisms Transfected cells Stem cells Physical fractionation centrifugal elutriation of apoptotic cells nuclei of mammalian cells large scale mitotic cells chromosome formation fertilization engraftment in transplantation

Editors and affiliations

  • Gaspar Banfalvi
    • 1
  1. 1.Department of Biotechnology and MicrobiologyUniversity of DebrecenDebrecenHungary

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6603-5
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6602-8
  • Online ISBN 978-1-4939-6603-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book