Abstract
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using biotinylated antisense oligonucleotides that hybridize to the target RNA. Co-precipitated DNA is then purified and analyzed by quantitative PCR (qPCR) or high-throughput sequencing.
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Acknowledgement
This work was supported by the Singapore Agency for Science, Technology, and Research (A*STAR to C.C.) and the US National Institutes of Healthy (H.Y.C.). H.Y.C. is an Early Career Scientist of the Howard Hughes Medical Institute.
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Chu, C., Chang, H.Y. (2016). Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP). In: Lanzuolo, C., Bodega, B. (eds) Polycomb Group Proteins. Methods in Molecular Biology, vol 1480. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6380-5_10
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DOI: https://doi.org/10.1007/978-1-4939-6380-5_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6380-5
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