Abstract
Hydrotropism is a genuine response of roots to a moisture gradient to avoid drought. An experimental system for the induction of hydrotropic root response in petri dishes was designed by pioneering groups in the field. This system uses split agar plates containing an osmolyte only in a region of the plate in order to generate a water potential gradient. Arabidopsis seedlings are placed on the MS agar plate so that their root tips are near the junction between plain MS medium and the region supplemented with the osmolyte. This elicits a hydrotropic response in Arabidopsis roots that can be measured as the root curvature angle.
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References
Verslues PE, Agarwal M, Katiyar-Agarwal S et al (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:523–539
Roy R, Bassham DC (2014) Root growth movements: waving and skewing. Plant Sci 221:42–47
Jaffe MJ, Takahashi H, Biro RL (1985) A pea mutant for the study of hydrotropism in roots. Science 230:445–447
Eapen D, Barroso ML, Campos ME et al (2003) A no hydrotropic response (nhr1) root mutant that responds positively to gravitropism in Arabidopsis. Plant Physiol 131:536–546
Kobayashi A, Takahashi A, Kakimoto Y et al (2007) A gene essential for hydrotropism in roots. Proc Natl Acad Sci USA 104:4724–4729
Takahashi N, Goto N, Okada K et al (2002) Hydrotropism in abscisic acid, wavy, and gravitropic mutants of Arabidopsis thaliana. Planta 216:203–211
Miyazawa Y, Takahashi A, Kobayashi A et al (2009) GNOM-mediated vesicular trafficking plays an essential role in hydrotropism of Arabidopsis roots. Plant Physiol 149:835–840
Saucedo M, Ponce G, Campos M et al (2012) An altered hydrotropic response (ahr1) mutant of Arabidopsis recovers root hydrotropism with cytokinin. J Exp Bot 63:3587–3601
Antoni R, Gonzalez-Guzman M, Rodriguez L et al (2013) PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root. Plant Physiol 161:931–941
Murashige T, Skoog F (1962) A revised medium for rapid growth and Bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Wells DM, French AP, Naeem A et al (2012) Recovering the dynamics of root growth and development using novel image acquisition and analysis methods. Philos Trans R Soc B: Biol Sci 367:2245
Moriwaki T, Miyazawa Y, Fujii N et al (2012) Light and abscisic acid signalling are integrated by MIZ1 gene expression and regulate hydrotropic response in roots of Arabidopsis thaliana. Plant Cell Environ 35:1359–1368
Acknowledgment
Funding was provided by a Marie Curie Intra-European Fellowship to R.A. and grants from the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas to P.L.R. (BIO2014-52537-R) and the Biotechnology and Biological Sciences Research Council to D.D. and M.J.B.
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Antoni, R., Dietrich, D., Bennett, M.J., Rodriguez, P.L. (2016). Hydrotropism: Analysis of the Root Response to a Moisture Gradient. In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_1
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DOI: https://doi.org/10.1007/978-1-4939-3356-3_1
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