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Methods for PTEN in Stem Cells and Cancer Stem Cells

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PTEN

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1388))

Abstract

PTEN (phosphatase and tensin homologue) is the first tumor suppressor identified to have phosphatase activity and its gene is the second most frequently deleted or mutated tumor-suppressor gene associated with human cancers. Germline PTEN mutations are the cause of three inherited autosomal dominant disorders. Phosphatidylinositol 3,4,5,-triphosphate (PIP3), the product of the PI3 kinase, is one of the key intracellular targets of PTEN’s phosphatase activity, although PTEN’s phosphatase-independent activities have also been identified. PTEN is critical for stem cell maintenance, which contributes to its controlled tumorigenesis. PTEN loss leads the development of cancer stem cells (CSCs) that share properties with somatic stem cells, including the capacity for self-renewal and multi-lineage differentiation. Methods to isolate and functionally test stem cells and CSCs are important for understanding PTEN functions and the development of therapeutic approaches to target CSCs without having adverse effects on normal stem cells. Here, we describe protocols for the isolation and functional analysis of PTEN deficient embryonic stem cells, hematopoietic stem cells and leukemia-initiating cells (LICs), neural stem cells, and prostate stem cells and CSCs.

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Authors and Affiliations

  1. Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, CA, USA

    Suzanne Schubbert, Jing Jiao, Marcus Ruscetti, Jonathan Nakashima, Shumin Wu & Hong Wu

  2. The MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 5 Yheyuan Road, Beijing, 100871, China

    Hong Lei, Qinzhi Xu, Wenkai Yi, Haichuan Zhu & Hong Wu

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  1. Suzanne Schubbert
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  2. Jing Jiao
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  3. Marcus Ruscetti
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  4. Jonathan Nakashima
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  5. Shumin Wu
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  6. Hong Lei
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  7. Qinzhi Xu
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  8. Wenkai Yi
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  9. Haichuan Zhu
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  10. Hong Wu
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Correspondence to Hong Wu .

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Editors and Affiliations

  1. University of Toronto, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

    Leonardo Salmena

  2. University of Toronto, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

    Vuk Stambolic

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Schubbert, S. et al. (2016). Methods for PTEN in Stem Cells and Cancer Stem Cells. In: Salmena, L., Stambolic, V. (eds) PTEN. Methods in Molecular Biology, vol 1388. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3299-3_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3299-3_15

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