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Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation

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Recombinant Proteins from Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1385))

Abstract

Nicotiana benthamiana has shown great success as a platform for the production of recombinant proteins. Here, we describe methods to transiently express high levels of recombinant proteins and simultaneously modulate their glycosylation pattern toward human-like structures. The method aims to generate recombinant proteins with a targeted largely homogeneous glycosylation profile for structure–function studies.

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Acknowledgments

This work was supported by a grant from the Austrian Research Promotion Agency (FFG) Laura Bassi Centre of Expertise PlantBioP (Number 822757), and the Austrian Science Fund (FWF; Grant Number L575-B13).

Author information

Authors and Affiliations

  1. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria

    Alexandra Castilho & Herta Steinkellner

Authors
  1. Alexandra Castilho
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  2. Herta Steinkellner
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Corresponding author

Correspondence to Herta Steinkellner .

Editor information

Editors and Affiliations

  1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada

    Jacqueline MacDonald

  2. Department of Biology, Western University, London, Ontario, Canada

    Igor Kolotilin

  3. London, Ontario, Canada

    Rima Menassa

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Castilho, A., Steinkellner, H. (2016). Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3289-4_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3288-7

  • Online ISBN: 978-1-4939-3289-4

  • eBook Packages: Springer Protocols

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