Abstract
A multiple reaction monitoring (MRM), positive ion electrospray ionization, LC/MS/MS method is described for the quantification of cetirizine. The compound was isolated from human plasma by protein precipitation using acetonitrile. Cetirizine d4 was used as an internal standard. Chromatographic conditions were achieved using a C18 column and a combination of ammonium acetate, water, and methanol as the mobile phase. MRMs were: cetirizine, 389.26 → 165.16, 201.09; cetirizine d4, 393.09 → 165.15, 201.10. Calibration curves were constructed by plotting the peak area ratios of the calibrators’ target MRM transition area to labeled internal standard target MRM transition area versus concentration.
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References
Bachert C (2005) Levocetirizine: a modern H1-antihistamine for the treatment of allergic rhinitis. Expert Rev Clin Immunol 1:495–510
Kapp A, Wedi B (2004) Chronic urticaria: clinical aspects and focus on a new antihistamine, levocetirizine. J Drugs Dermatol 3:632–639
Kranke B, Mayr-Kanhauser S (2005) Urticarial reaction to the antihistamine levocetirizine dihydrochloride. Dermatology 210:246–247
Walsh GM (2008) A review of the role of levocetirizine as an effective therapy for allergic disease. Expert Opin Pharmacother 9:859–867
Passalacqua G, Canonica GW (2005) A review of the evidence from comparative studies of levocetirizine and desloratadine for the symptoms of allergic rhinitis. Clin Ther 27:979–992
Morita MR, Berton D, Boldin R, Barros FA, Meurer EC, Amarante AR, Campos DR, Calafatti SA, Pereira R, Abib E Jr et al (2008) Determination of levocetirizine in human plasma by liquid chromatography-electrospray tandem mass spectrometry: application to a bioequivalence study. J Chromatogr B Analyt Technol Biomed Life Sci 862:132–139
Rosseel MT, Lefebvre RA (1991) Determination of cetirizine in human urine by high-performance liquid chromatography. J Chromatogr 565:504–510
Ren XL, Tian Y, Zhang ZJ, Chen Y, Wu LL, Huang J (2011) Determination of cetirizine in human plasma using high performance liquid chromatography coupled with tandem mass spectrometric detection: application to a bioequivalence study. Arzneimittelforschung 61:287–295
Pandya KK, Bangaru RA, Gandhi TP, Modi IA, Modi RI, Chakravarthy BK (1996) High-performance thin-layer chromatography for the determination of cetirizine in human plasma and its use in pharmacokinetic studies. J Pharm Pharmacol 48:510–513
Moncrieff J (1992) Determination of cetirizine in serum using reversed-phase high-performance liquid chromatography with ultraviolet spectrophotometric detection. J Chromatogr 583:128–130
Macek J, Ptacek P, Klima J (1999) Determination of cetirizine in human plasma by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 736:231–235
Kang SW, Jang HJ, Moore VS, Park JY, Kim KA, Youm JR, Han SB (2010) Enantioselective determination of cetirizine in human plasma by normal-phase liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 878:3351–3357
de Jager AD, Hundt HK, Swart KJ, Hundt AF, Els J (2002) Extractionless and sensitive method for high-throughput quantitation of cetirizine in human plasma samples by liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 773:113–118
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Munar, A., Frazee, C., Jones, B., Garg, U. (2016). Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 1383. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3252-8_13
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DOI: https://doi.org/10.1007/978-1-4939-3252-8_13
Publisher Name: Humana Press, New York, NY
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