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Quantification of Amino Acids in Plasma by High-Performance Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS)

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Abstract

For the extraction and measurement of plasma amino acids using LC/ESI–MS/MS technology, a straightforward and dependable approach has been established. The approach employs a small amount of sample and fully quantifies it using a labeled internal standard of amino acids. Water, formic acid, and methanol were used as the mobile phase in a gradient method to obtain clear separation. On a Shimatzu mass spectrometer 8030, optimized multiple reaction monitoring (MRM) was utilized to find amino acids. All amino acids have coefficients of correlation that range from 0.91 to 0.99, and they are all linear over their respective reference ranges. Precision’s intra-day and inter-day coefficients of variation (CV %) ranged from 3.29 to 11.73% and 5.04 to 12.48%, respectively. Amino acid recovery ranged from 92.1 to 108.2%.

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Data availability

The data that support the findings of this study are available on request from the corresponding author (shrimant.p@gd-lab.com).

Abbreviations

HPLC:

High-performance liquid chromatography

GCMS:

Gas chromatography–mass spectrometry

IEM:

Inborn error of metabolism

TMS:

Tandem mass spectrometry

ESI:

Electrospray ionization

µL:

Micro liter

µM·L-1 :

Micromole per liter

MRM:

Multiple reaction monitoring

RT:

Retention time

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Acknowledgements

We are grateful to Mr. Abhimanyu Kumar, CEO and Mr. Susheel Sing CTO of General Diagnostics International Pvt Ltd, Mumbai (India), for their institutional funding, continuing interest, encouragement, and administrative support.

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The authors declare that they have no any external agency funding for the present work.

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Authors and Affiliations

  1. General Diagnostics International (P) Ltd, Navi Mumbai, Maharashtra, India

    Shrimant N. Panaskar & Susheel K. Singh

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  1. Shrimant N. Panaskar
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  2. Susheel K. Singh
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All authors equally contributed to the study conception and design, material preparation, data collection, and analysis.

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Correspondence to Shrimant N. Panaskar.

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Panaskar, S.N., Singh, S.K. Quantification of Amino Acids in Plasma by High-Performance Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS). Chromatographia 86, 567–572 (2023). https://doi.org/10.1007/s10337-023-04262-3

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