Abstract
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid–detergent concentrations (“HiLiDe”) circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein–lipid–detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Toyoshima C, Nakasako M, Nomura H, Ogawa H (2000) Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution. Nature 405:647
Morth JP et al (2007) Crystal structure of the sodium-potassium pump. Nature 450:1043
Shinoda T, Ogawa H, Cornelius F, Toyoshima C (2009) Crystal structure of the sodium-potassium pump at 2.4 A resolution. Nature 459:446
Pedersen BP, Buch-Pedersen MJ, Morth JP, Palmgren MG, Nissen P (2007) Crystal structure of the plasma membrane proton pump. Nature 450:1111
Gourdon P et al (2011) Crystal structure of a copper-transporting PIB-type ATPase. Nature 475:59
Wang K et al (2014) Structure and mechanism of Zn-transporting P-type ATPases. Nature 514:518–522
Newby ZE et al (2009) A general protocol for the crystallization of membrane proteins for X-ray structural investigation. Nat Protoc 4:619
Bowie JU (2001) Stabilizing membrane proteins. Curr Opin Struct Biol 11:397
Caffrey M, Cherezov V (2009) Crystallizing membrane proteins using lipidic mesophases. Nat Protoc 4:706
Johansson LC, Wohri AB, Katona G, Engstrom S, Neutze R (2009) Membrane protein crystallization from lipidic phases. Curr Opin Struct Biol 19:372
Gourdon P et al (2011) HiLiDe-systematic approach to membrane protein crystallization in lipid and detergent. Cryst Growth Des 11:2098
Bublitz M, Poulsen H, Morth JP, Nissen P (2010) In and out of the cation pumps: P-Type ATPase structure revisited. Curr Opin Struct Biol 20:431
Sorensen TL, Olesen C, Jensen AM, Moller JV, Nissen P (2006) Crystals of sarcoplasmic reticulum Ca(2+)-ATPase. J Biotechnol 124:704
Acknowledgements
This work was supported by the Graduate School of Science and Technology at Aarhus University and by grants to P.G. from the Swedish Research Council and the The Lundbeck Foundation.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Sitsel, O., Wang, K., Liu, X., Gourdon, P. (2016). Crystallization of P-type ATPases by the High Lipid–Detergent (HiLiDe) Method. In: Bublitz, M. (eds) P-Type ATPases. Methods in Molecular Biology, vol 1377. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3179-8_37
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3179-8_37
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3178-1
Online ISBN: 978-1-4939-3179-8
eBook Packages: Springer Protocols