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Analysis of Genotyping-by-Sequencing (GBS) Data

  • Protocol
Plant Bioinformatics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1374))

Abstract

The development of genotyping-by-sequencing (GBS) to rapidly detect nucleotide variation at the whole genome level, in many individuals simultaneously, has provided a transformative genetic profiling technique. GBS can be carried out in species with or without reference genome sequences yields huge amounts of potentially informative data. One limitation with the approach is the paucity of tools to transform the raw data into a format that can be easily interrogated at the genetic level. In this chapter we describe bioinformatics tools developed to address this shortfall together with experimental design considerations to fully leverage the power of GBS for genetic analysis.

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Author information

Authors and Affiliations

  1. National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, Canada, S7N 0W9

    Sateesh Kagale, Chushin Koh & Andrew G. Sharpe

  2. Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, Canada, S7N 0X2

    Wayne E. Clarke, Venkatesh Bollina & Isobel A. P. Parkin

Authors
  1. Sateesh Kagale
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  2. Chushin Koh
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  3. Wayne E. Clarke
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  4. Venkatesh Bollina
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  5. Isobel A. P. Parkin
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  6. Andrew G. Sharpe
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Corresponding author

Correspondence to Andrew G. Sharpe .

Editor information

Editors and Affiliations

  1. School of Plant Biology, University of Western Australia, Perth, Australia

    David Edwards

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© 2016 Springer Science+Business Media New York

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Kagale, S., Koh, C., Clarke, W.E., Bollina, V., Parkin, I.A.P., Sharpe, A.G. (2016). Analysis of Genotyping-by-Sequencing (GBS) Data. In: Edwards, D. (eds) Plant Bioinformatics. Methods in Molecular Biology, vol 1374. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3167-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3167-5_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3166-8

  • Online ISBN: 978-1-4939-3167-5

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