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Dendrimer Nanovectors for SiRNA Delivery

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SiRNA Delivery Methods

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1364))

Abstract

Small interfering RNA (SiRNA) delivery remains a major challenge in RNAi-based therapy. Dendrimers are emerging as appealing nonviral vectors for SiRNA delivery thanks to their well-defined architecture and their unique cooperativity and multivalency confined within a nanostructure. We have recently demonstrated that structurally flexible poly(amidoamine) (PAMAM) dendrimers are safe and effective nanovectors for SiRNA delivery in various disease models in vitro and in vivo. The present chapter showcases these dendrimers can package different SiRNA molecules into stable and nanosized particles, which protect SiRNA from degradation and promote cellular uptake of SiRNA, resulting in potent gene silencing at both mRNA and protein level in the prostate cancer cell model. Our results demonstrate this set of flexible PAMAM dendrimers are indeed safe and effective nonviral vectors for SiRNA delivery and hold great promise for further applications in functional genomics and RNAi-based therapies.

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Acknowledgments

This work was supported by the international ERA-Net EURONANOMED European Research project DENANORNA, PACA Canceropôle, INCa, Aix-Marseille Université, CNRS, INSERM, China Scholarship Council (XL), Association pour la Recherche sur les Tumeurs de la Prostate (XL), Association Française contre les Myopathies (XL), and under the auspice of European COST Action TD0802 “Dendrimers in Biomedical Applications.”

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Authors and Affiliations

  1. Aix-Marseille Université, CNRS CINaM UMR 7325, 163 avenue de Luminy, 13288, Marseille Cedex 09, France

    Xiaoxuan Liu & Ling Peng

Authors
  1. Xiaoxuan Liu
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  2. Ling Peng
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Corresponding author

Correspondence to Ling Peng .

Editor information

Editors and Affiliations

  1. Beckman Research Institute of City of Hope, Duarte, California, USA

    Kato Shum

  2. Beckman Research Institute of City of Hope, Duarte, California, USA

    John Rossi

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Liu, X., Peng, L. (2016). Dendrimer Nanovectors for SiRNA Delivery. In: Shum, K., Rossi, J. (eds) SiRNA Delivery Methods. Methods in Molecular Biology, vol 1364. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3112-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-3112-5_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3111-8

  • Online ISBN: 978-1-4939-3112-5

  • eBook Packages: Springer Protocols

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